Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5350780..5351405 Replicon chromosome
Accession NZ_CP072996
Organism Klebsiella pneumoniae strain KP1878

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KA532_RS26385 Protein ID WP_002882817.1
Coordinates 5350780..5351163 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KA532_RS26390 Protein ID WP_004150355.1
Coordinates 5351163..5351405 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KA532_RS26370 (KA532_26370) 5348146..5349048 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KA532_RS26375 (KA532_26375) 5349045..5349680 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KA532_RS26380 (KA532_26380) 5349677..5350606 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KA532_RS26385 (KA532_26385) 5350780..5351163 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KA532_RS26390 (KA532_26390) 5351163..5351405 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KA532_RS26395 (KA532_26395) 5351610..5352527 + 918 WP_002882812.1 alpha/beta hydrolase -
KA532_RS26400 (KA532_26400) 5352541..5353482 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KA532_RS26405 (KA532_26405) 5353527..5353964 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KA532_RS26410 (KA532_26410) 5353961..5354821 - 861 WP_002882807.1 virulence factor BrkB family protein -
KA532_RS26415 (KA532_26415) 5354815..5355414 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199450 WP_002882817.1 NZ_CP072996:c5351163-5350780 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199450 NZ_CP097346:c5031-4615 [Enterobacter hormaechei subsp. xiangfangensis]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199450 WP_004150355.1 NZ_CP072996:c5351405-5351163 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199450 NZ_CP097346:c5258-5028 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References