Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4767263..4768073 Replicon chromosome
Accession NZ_CP072996
Organism Klebsiella pneumoniae strain KP1878

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KA532_RS23590 Protein ID WP_002887280.1
Coordinates 4767263..4767796 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KA532_RS23595 Protein ID WP_002887278.1
Coordinates 4767807..4768073 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KA532_RS23585 (KA532_23590) 4766094..4767215 + 1122 WP_002887282.1 cupin domain-containing protein -
KA532_RS23590 (KA532_23595) 4767263..4767796 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KA532_RS23595 (KA532_23600) 4767807..4768073 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KA532_RS23600 (KA532_23605) 4768176..4769609 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KA532_RS23605 (KA532_23610) 4769599..4770282 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KA532_RS23610 (KA532_23615) 4770454..4771839 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KA532_RS23615 (KA532_23620) 4771857..4772201 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T199447 WP_002887280.1 NZ_CP072996:c4767796-4767263 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T199447 NZ_CP097342:c4730504-4730133 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT199447 WP_002887278.1 NZ_CP072996:c4768073-4767807 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT199447 NZ_CP097342:c4730749-4730507 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References