Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4154574..4155193 Replicon chromosome
Accession NZ_CP072996
Organism Klebsiella pneumoniae strain KP1878

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KA532_RS20645 Protein ID WP_002892050.1
Coordinates 4154975..4155193 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KA532_RS20640 Protein ID WP_002892066.1
Coordinates 4154574..4154948 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KA532_RS20630 (KA532_20635) 4149726..4150919 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KA532_RS20635 (KA532_20640) 4150942..4154088 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
KA532_RS20640 (KA532_20645) 4154574..4154948 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KA532_RS20645 (KA532_20650) 4154975..4155193 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KA532_RS20650 (KA532_20655) 4155352..4155918 + 567 WP_002892030.1 maltose O-acetyltransferase -
KA532_RS20655 (KA532_20660) 4155890..4156030 - 141 WP_004147370.1 hypothetical protein -
KA532_RS20660 (KA532_20665) 4156051..4156521 + 471 WP_002892026.1 YlaC family protein -
KA532_RS20665 (KA532_20670) 4156496..4157947 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KA532_RS20670 (KA532_20675) 4158048..4158746 + 699 WP_002892021.1 GNAT family protein -
KA532_RS20675 (KA532_20680) 4158743..4158883 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KA532_RS20680 (KA532_20685) 4158883..4159146 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199446 WP_002892050.1 NZ_CP072996:4154975-4155193 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T199446 NZ_CP097342:c4607053-4606868 [Enterobacter hormaechei subsp. xiangfangensis]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAACCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT199446 WP_002892066.1 NZ_CP072996:4154574-4154948 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT199446 NZ_CP097342:c4606853-4606449 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTGCCTCTCCCGGGTAGTGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACAGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References