Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
Location | 95293..95870 | Replicon | plasmid unnamed |
Accession | NZ_CP072987 | ||
Organism | Escherichia coli strain T159A |
Toxin (Protein)
Gene name | mazF | Uniprot ID | U5N651 |
Locus tag | KBW97_RS23075 | Protein ID | WP_023159957.1 |
Coordinates | 95293..95625 (-) | Length | 111 a.a. |
Antitoxin (Protein)
Gene name | mazE | Uniprot ID | U5N340 |
Locus tag | KBW97_RS23080 | Protein ID | WP_023159984.1 |
Coordinates | 95625..95870 (-) | Length | 82 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
KBW97_RS23045 | 91134..91952 | - | 819 | WP_023159955.1 | DUF4365 domain-containing protein | - |
KBW97_RS23050 | 91966..92919 | - | 954 | WP_023159956.1 | site-specific integrase | - |
KBW97_RS23055 | 93214..93483 | + | 270 | WP_048821758.1 | hypothetical protein | - |
KBW97_RS23060 | 93875..94333 | + | 459 | WP_048821762.1 | hypothetical protein | - |
KBW97_RS23065 | 94653..94793 | + | 141 | WP_072022937.1 | Hok/Gef family protein | - |
KBW97_RS23070 | 94882..95262 | - | 381 | WP_048821763.1 | hypothetical protein | - |
KBW97_RS23075 | 95293..95625 | - | 333 | WP_023159957.1 | endoribonuclease MazF | Toxin |
KBW97_RS23080 | 95625..95870 | - | 246 | WP_023159984.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
KBW97_RS23085 | 96147..96770 | + | 624 | WP_023159958.1 | AAA family ATPase | - |
KBW97_RS23090 | 96879..97106 | + | 228 | WP_048821767.1 | plasmid partition protein ParG | - |
KBW97_RS23095 | 97204..97572 | - | 369 | WP_048821769.1 | hypothetical protein | - |
KBW97_RS23100 | 98333..98911 | - | 579 | WP_048822182.1 | hypothetical protein | - |
KBW97_RS23105 | 98920..99399 | - | 480 | WP_048821772.1 | Hcp family type VI secretion system effector | - |
KBW97_RS23110 | 99972..100211 | + | 240 | WP_001353740.1 | DUF1819 family protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / floR / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / aadA14 / lnu(G) / dfrA1 | - | 1..104812 | 104812 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 12097.88 Da Isoelectric Point: 7.0813
>T199425 WP_023159957.1 NZ_CP072987:c95625-95293 [Escherichia coli]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG
Download Length: 333 bp
>T199425 NZ_CP097339:c606515-606246 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA
Antitoxin
Download Length: 82 a.a. Molecular weight: 8786.20 Da Isoelectric Point: 5.0351
>AT199425 WP_023159984.1 NZ_CP072987:c95870-95625 [Escherichia coli]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I
Download Length: 246 bp
>AT199425 NZ_CP097339:c606785-606519 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2P1BQ75 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A857E7M9 |