Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 95293..95870 Replicon plasmid unnamed
Accession NZ_CP072987
Organism Escherichia coli strain T159A

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag KBW97_RS23075 Protein ID WP_023159957.1
Coordinates 95293..95625 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag KBW97_RS23080 Protein ID WP_023159984.1
Coordinates 95625..95870 (-) Length 82 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW97_RS23045 91134..91952 - 819 WP_023159955.1 DUF4365 domain-containing protein -
KBW97_RS23050 91966..92919 - 954 WP_023159956.1 site-specific integrase -
KBW97_RS23055 93214..93483 + 270 WP_048821758.1 hypothetical protein -
KBW97_RS23060 93875..94333 + 459 WP_048821762.1 hypothetical protein -
KBW97_RS23065 94653..94793 + 141 WP_072022937.1 Hok/Gef family protein -
KBW97_RS23070 94882..95262 - 381 WP_048821763.1 hypothetical protein -
KBW97_RS23075 95293..95625 - 333 WP_023159957.1 endoribonuclease MazF Toxin
KBW97_RS23080 95625..95870 - 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KBW97_RS23085 96147..96770 + 624 WP_023159958.1 AAA family ATPase -
KBW97_RS23090 96879..97106 + 228 WP_048821767.1 plasmid partition protein ParG -
KBW97_RS23095 97204..97572 - 369 WP_048821769.1 hypothetical protein -
KBW97_RS23100 98333..98911 - 579 WP_048822182.1 hypothetical protein -
KBW97_RS23105 98920..99399 - 480 WP_048821772.1 Hcp family type VI secretion system effector -
KBW97_RS23110 99972..100211 + 240 WP_001353740.1 DUF1819 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / floR / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / aadA14 / lnu(G) / dfrA1 - 1..104812 104812


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-107)

Antitoxin

(7-49)


Sequences


Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T199425 WP_023159957.1 NZ_CP072987:c95625-95293 [Escherichia coli]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T199425 NZ_CP097339:c606515-606246 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT199425 WP_023159984.1 NZ_CP072987:c95870-95625 [Escherichia coli]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT199425 NZ_CP097339:c606785-606519 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75


Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References