Detailed information of TA system TA199422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4007756..4008570
• Replicon: chromosome
• Accession: NZ_CP072986
• Organism: Escherichia coli strain T159A

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: KBW97_RS19500
• Protein ID: WP_001054376.1
• Coordinates: 4007756..4008013 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: KBW97_RS19505
• Protein ID: WP_001309181.1
• Coordinates: 4008025..4008570 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW97_RS19480 (4003044)	4003044..4004150	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
KBW97_RS19485 (4004215)	4004215..4005195	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
KBW97_RS23370 (4005305)	4005305..4005510	+	206	Protein_3857	HNH endonuclease	-
KBW97_RS19490 (4005778)	4005778..4007018	-	1241	Protein_3858	helicase YjhR	-
KBW97_RS23375 (4007134)	4007134..4007265	+	132	WP_001309182.1	hypothetical protein	-
KBW97_RS19500 (4007756)	4007756..4008013	+	258	WP_001054376.1	YjhX family toxin	Toxin
KBW97_RS19505 (4008025)	4008025..4008570	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
KBW97_RS19510 (4008626)	4008626..4009372	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
KBW97_RS19515 (4009541)	4009541..4009759	+	219	Protein_3863	hypothetical protein	-
KBW97_RS19520 (4009797)	4009797..4009913	+	117	Protein_3864	VOC family protein	-
KBW97_RS19525 (4010158)	4010158..4011279	+	1122	WP_000010829.1	M42 family metallopeptidase	-
KBW97_RS19530 (4011276)	4011276..4011554	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
KBW97_RS19535 (4011566)	4011566..4012879	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3996766..4016794	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T199422 WP_001054376.1 NZ_CP072986:4007756-4008013 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T199422 NZ_CP097339:c15354-15022 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT199422 WP_001309181.1 NZ_CP072986:4008025-4008570 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT199422 NZ_CP097339:c15611-15351 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8