Detailed information of TA system TA199415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2377422..2378060
• Replicon: chromosome
• Accession: NZ_CP072986
• Organism: Escherichia coli strain T159A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KBW97_RS11450
• Protein ID: WP_000813794.1
• Coordinates: 2377884..2378060 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KBW97_RS11445
• Protein ID: WP_001270286.1
• Coordinates: 2377422..2377838 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW97_RS11425 (2372574)	2372574..2373515	-	942	WP_001251304.1	ABC transporter permease	-
KBW97_RS11430 (2373516)	2373516..2374529	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KBW97_RS11435 (2374547)	2374547..2375692	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KBW97_RS11440 (2375937)	2375937..2377343	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KBW97_RS11445 (2377422)	2377422..2377838	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KBW97_RS11450 (2377884)	2377884..2378060	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KBW97_RS11455 (2378282)	2378282..2378512	+	231	WP_000494244.1	YncJ family protein	-
KBW97_RS11460 (2378604)	2378604..2380565	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KBW97_RS11465 (2380638)	2380638..2381174	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KBW97_RS11470 (2381227)	2381227..2382441	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T199415 WP_000813794.1 NZ_CP072986:c2378060-2377884 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T199415 NZ_CP097338:395101-395370 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT199415 WP_001270286.1 NZ_CP072986:c2377838-2377422 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT199415 NZ_CP097338:394848-395114 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Toxin

• PDB: 6HPB