Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 847929..848583 Replicon chromosome
Accession NZ_CP072986
Organism Escherichia coli strain T159A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KBW97_RS04085 Protein ID WP_000244777.1
Coordinates 848176..848583 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KBW97_RS04080 Protein ID WP_000354046.1
Coordinates 847929..848195 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW97_RS04055 (843098) 843098..843841 + 744 WP_000951964.1 SDR family oxidoreductase -
KBW97_RS04060 (843898) 843898..845331 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KBW97_RS04065 (845376) 845376..845687 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KBW97_RS04070 (845851) 845851..846510 + 660 WP_000250274.1 hemolysin III family protein -
KBW97_RS04075 (846706) 846706..847686 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KBW97_RS04080 (847929) 847929..848195 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KBW97_RS04085 (848176) 848176..848583 + 408 WP_000244777.1 protein YgfX Toxin
KBW97_RS04090 (848623) 848623..849144 - 522 WP_001055874.1 flavodoxin FldB -
KBW97_RS04095 (849256) 849256..850152 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KBW97_RS04100 (850177) 850177..850887 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KBW97_RS04105 (850893) 850893..852626 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T199399 WP_000244777.1 NZ_CP072986:848176-848583 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199399 NZ_CP097336:844118-844387 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGCAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATTGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199399 WP_000354046.1 NZ_CP072986:847929-848195 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199399 NZ_CP097336:843848-844114 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References