Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 69497..70098 Replicon plasmid pEC2547-3
Accession NZ_CP072982
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag KBW66_RS26335 Protein ID WP_001216034.1
Coordinates 69718..70098 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KBW66_RS26330 Protein ID WP_001190712.1
Coordinates 69497..69718 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS26310 (65117) 65117..66286 - 1170 Protein_83 IS3-like element ISEc52 family transposase -
KBW66_RS26315 (66573) 66573..67466 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -
KBW66_RS26325 (68355) 68355..69314 - 960 Protein_86 type I restriction-modification system subunit M -
KBW66_RS26330 (69497) 69497..69718 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KBW66_RS26335 (69718) 69718..70098 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KBW66_RS26340 (70103) 70103..70282 + 180 WP_001513661.1 hypothetical protein -
KBW66_RS26345 (70310) 70310..70588 + 279 Protein_90 pdcB -
KBW66_RS26350 (70593) 70593..71006 + 414 Protein_91 integrase core domain-containing protein -
KBW66_RS26355 (70956) 70956..71291 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
KBW66_RS26360 (71501) 71501..72481 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
KBW66_RS26365 (72725) 72725..74128 + 1404 WP_001373486.1 S-methylmethionine permease -
KBW66_RS26370 (74115) 74115..75047 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / tet(A) / blaCTX-M-27 / sul2 - 1..81323 81323
- inside IScluster/Tn sul2 - 65139..80705 15566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T199393 WP_001216034.1 NZ_CP072982:69718-70098 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T199393 NZ_CP097335:c1507309-1507013 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTATTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAACA
CGGTATTGATTTTGAACAGGCAAAGCTGCTTTGGGAAGATGAACGAAAAGTCGTATTGGAGGCTTGTACCGAACCTGAAT
TACGCTATTTCGCGATTGGTAAGATTTATGATAAATATTGGACAGCATTTTTTACTCCAAGAAATGATATTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATTTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT199393 WP_001190712.1 NZ_CP072982:69497-69718 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT199393 NZ_CP097335:c1507029-1506787 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCTGAAGAATTAGAGCGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGACCT
CTCCACCGCTCATCGCCCTGGGCAGATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTGGAAGCGTTAGATAAGC
AAGCTAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGCTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References