Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 63049..63574 Replicon plasmid pEC2547-3
Accession NZ_CP072982
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag KBW66_RS26295 Protein ID WP_001159868.1
Coordinates 63049..63354 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KBW66_RS26300 Protein ID WP_000813634.1
Coordinates 63356..63574 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS26280 (58959) 58959..60125 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KBW66_RS26285 (60713) 60713..61468 - 756 WP_000852146.1 replication initiation protein RepE -
KBW66_RS26290 (62242) 62242..63048 - 807 WP_000016982.1 site-specific integrase -
KBW66_RS26295 (63049) 63049..63354 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
KBW66_RS26300 (63356) 63356..63574 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KBW66_RS26305 (64282) 64282..65142 + 861 WP_251365050.1 nucleotidyltransferase -
KBW66_RS26310 (65117) 65117..66286 - 1170 Protein_83 IS3-like element ISEc52 family transposase -
KBW66_RS26315 (66573) 66573..67466 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / tet(A) / blaCTX-M-27 / sul2 - 1..81323 81323
- inside IScluster/Tn sul2 - 65139..80705 15566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T199392 WP_001159868.1 NZ_CP072982:c63354-63049 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T199392 NZ_CP097335:c789569-789372 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACATTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCCCCCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT199392 WP_000813634.1 NZ_CP072982:c63574-63356 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT199392 NZ_CP097335:c789303-788974 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCAGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References