Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5092354..5093008 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag KBW66_RS24800 Protein ID WP_000244765.1
Coordinates 5092354..5092761 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag KBW66_RS24805 Protein ID WP_000354050.1
Coordinates 5092742..5093008 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS24780 (5088311) 5088311..5090044 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
KBW66_RS24785 (5090050) 5090050..5090760 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KBW66_RS24790 (5090785) 5090785..5091681 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KBW66_RS24795 (5091793) 5091793..5092314 + 522 WP_001055874.1 flavodoxin FldB -
KBW66_RS24800 (5092354) 5092354..5092761 - 408 WP_000244765.1 protein YgfX Toxin
KBW66_RS24805 (5092742) 5092742..5093008 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
KBW66_RS24810 (5093251) 5093251..5094231 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
KBW66_RS24815 (5094308) 5094308..5094967 - 660 WP_000250275.1 hemolysin III family protein -
KBW66_RS24820 (5095131) 5095131..5095442 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KBW66_RS24825 (5095487) 5095487..5096920 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T199386 WP_000244765.1 NZ_CP072980:c5092761-5092354 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199386 NZ_CP097334:c1102157-1101975 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT199386 WP_000354050.1 NZ_CP072980:c5093008-5092742 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199386 NZ_CP097334:c1101936-1101517 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References