Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4145061..4145892 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag KBW66_RS20535 Protein ID WP_000854815.1
Coordinates 4145518..4145892 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag KBW66_RS20530 Protein ID WP_001280918.1
Coordinates 4145061..4145429 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS20485 (4140150) 4140150..4140896 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
KBW66_RS20490 (4140979) 4140979..4141329 + 351 Protein_4050 hypothetical protein -
KBW66_RS20495 (4141345) 4141345..4141755 + 411 WP_000846703.1 hypothetical protein -
KBW66_RS20500 (4141976) 4141976..4142794 + 819 WP_001542275.1 DUF932 domain-containing protein -
KBW66_RS20505 (4142794) 4142794..4143039 + 246 WP_001164966.1 hypothetical protein -
KBW66_RS20510 (4143133) 4143133..4143606 + 474 WP_001542276.1 antirestriction protein -
KBW66_RS20515 (4143622) 4143622..4144098 + 477 WP_001186200.1 RadC family protein -
KBW66_RS20520 (4144161) 4144161..4144382 + 222 WP_000692345.1 DUF987 domain-containing protein -
KBW66_RS20525 (4144401) 4144401..4145045 + 645 WP_000086752.1 hypothetical protein -
KBW66_RS20530 (4145061) 4145061..4145429 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KBW66_RS20535 (4145518) 4145518..4145892 + 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KBW66_RS20540 (4145889) 4145889..4146083 + 195 WP_000988600.1 DUF5983 family protein -
KBW66_RS20545 (4146129) 4146129..4146209 + 81 Protein_4061 hypothetical protein -
KBW66_RS26785 (4146498) 4146498..4146578 - 81 WP_023441679.1 hypothetical protein -
KBW66_RS26790 (4146557) 4146557..4146880 + 324 WP_225469267.1 EutP/PduV family microcompartment system protein -
KBW66_RS20560 (4146981) 4146981..4147310 - 330 WP_000450409.1 DUF496 family protein -
KBW66_RS20565 (4147482) 4147482..4148540 - 1059 WP_001200889.1 FUSC family protein -
KBW66_RS20570 (4148738) 4148738..4149211 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
KBW66_RS20575 (4149330) 4149330..4150496 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T199384 WP_000854815.1 NZ_CP072980:4145518-4145892 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T199384 NZ_CP097334:c861010-860786 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT199384 WP_001280918.1 NZ_CP072980:4145061-4145429 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT199384 NZ_CP097334:c860743-860414 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References