Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2373748..2374366 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KBW66_RS11415 Protein ID WP_001291435.1
Coordinates 2373748..2373966 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KBW66_RS11420 Protein ID WP_000344800.1
Coordinates 2373992..2374366 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS11380 (2369035) 2369035..2369607 + 573 WP_000779831.1 YbaY family lipoprotein -
KBW66_RS11385 (2369638) 2369638..2369949 - 312 WP_000409908.1 MGMT family protein -
KBW66_RS11395 (2370328) 2370328..2370681 + 354 WP_000878135.1 DUF1428 family protein -
KBW66_RS11400 (2370723) 2370723..2372273 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KBW66_RS11405 (2372437) 2372437..2372907 - 471 WP_000136192.1 YlaC family protein -
KBW66_RS11410 (2373023) 2373023..2373574 - 552 WP_000102539.1 maltose O-acetyltransferase -
KBW66_RS11415 (2373748) 2373748..2373966 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KBW66_RS11420 (2373992) 2373992..2374366 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KBW66_RS11425 (2374912) 2374912..2378061 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
KBW66_RS11430 (2378084) 2378084..2379277 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199369 WP_001291435.1 NZ_CP072980:c2373966-2373748 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T199369 NZ_CP097329:c4385167-4384811 [Pseudanabaena mucicola str. Chao 1806]
ATGAATATTCAACGCGGAGATATTGTGCTAGTCGATTATCCCTACACTTCGAGTAGTGAAACTAAAGTCCGACCAGTCCT
TGTAATTCAGAACGATCGCGACAACCAACGGCTCAAAAACACAATCGTAGTCCAAATCACAAGCCAAACCCAACGCTCCT
TAGAAACAACGCAGTTATTAATCAAGATGGCAACCTATGAGGGGCAAGAATCTGGATTAAGACAAGATTCTGTCATTAAC
TGCGTCAATCTACTTACGCTCAGTAAAGATAAAATACTTAGAAAATTGGGGCAGCTACCGAATTCCTCACTCCAAAAAGT
TAACGACTGTTTAAAGGCAGCACTGGAGTTAACCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT199369 WP_000344800.1 NZ_CP072980:c2374366-2373992 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT199369 NZ_CP097329:c4384778-4384560 [Pseudanabaena mucicola str. Chao 1806]
ATGAATGTATCCATCTCCATAGATTTCTCTCAACTTAAAACAGCGATCGCACAATGCAATCTAGAAGAAAAGCTGGAATT
ATTGCAGTTGCTAGAAAAAGACACATTTTCAGTGAGATTCAATAAATTTTTAAGTTCAGTACAAACTGACGAACTAAGTT
TAGAAGATATTACCCAAGAAGTTGAAGCCGTAAGGCAAGCAAATTATCATGCAAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References