Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1875505..1876025 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KBW66_RS09010 Protein ID WP_001160964.1
Coordinates 1875741..1876025 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KBW66_RS09005 Protein ID WP_000125566.1
Coordinates 1875505..1875738 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS08980 (1870578) 1870578..1870865 + 288 WP_000203744.1 ferredoxin-like protein FixX -
KBW66_RS08985 (1870924) 1870924..1872255 + 1332 WP_001183209.1 MFS transporter -
KBW66_RS08990 (1872363) 1872363..1872893 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KBW66_RS08995 (1872886) 1872886..1874748 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KBW66_RS09000 (1874940) 1874940..1875419 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KBW66_RS09005 (1875505) 1875505..1875738 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KBW66_RS09010 (1875741) 1875741..1876025 + 285 WP_001160964.1 CcdB family protein Toxin
KBW66_RS09015 (1876052) 1876052..1876900 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KBW66_RS09020 (1876907) 1876907..1877284 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KBW66_RS09025 (1877287) 1877287..1878108 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KBW66_RS09030 (1878105) 1878105..1879094 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KBW66_RS09035 (1879094) 1879094..1880380 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T199367 WP_001160964.1 NZ_CP072980:1875741-1876025 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T199367 NZ_CP097329:c4283649-4283260 [Pseudanabaena mucicola str. Chao 1806]
ATGGATACTCATATCTGGTTGTGGTGGTTTGCTCAGCCAGAGCGTTTGAGTGAAGAGGCGATCGCTTGCATAGTCGATGA
AACCAATGAGGTGTGGTTTTCAGTGGCAAGTGTCTGGGAAATGTCTATCAAAGTTGCGCTCGGCAAGTTGCCACTGCCAG
AACCAGTCGATAGTTATATCTCAAGTCGCATGGTGCAATTGGGAGCGCGATCGCTCGATATTTCAGTCAATCATGCCTTA
AAAGCTGCGGCTTTGCCTTTACATCATCGCGATCCATTTGATCGAATGTTAATCGCACAGGCTCAATTGGAAAATATAAC
GATTGTGACTGCTGATCATATGTTTAGCCAATATCAAGACGTTTCTATTTTGTGGGCTGCAAATTTATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT199367 WP_000125566.1 NZ_CP072980:1875505-1875738 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT199367 NZ_CP097329:c4283903-4283658 [Pseudanabaena mucicola str. Chao 1806]
ATGGAAACTGTTAATATTCATCAGGCTAAAACCAATCTTTCCCGACTTTTGGCAAGGGTTGAACTTGGAGAAGAGATTGT
TATTTCTAACCGAGGTGTTCCCGTTGCCAAGCTCGTTCCATTTTGTACTTCTTCTAATCGTAAAGCGAGTTTAGGCATAG
ATAAGGGACGCTTTATCGTGCCTGAAGATTTTAACGATCCTTTGCCAGAAGATATTTTGTCAGCATTTGAGGGTGGTGAA
GGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References