Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1765463..1766039 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag KBW66_RS08475 Protein ID WP_001295743.1
Coordinates 1765463..1765750 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag KBW66_RS08480 Protein ID WP_000063148.1
Coordinates 1765737..1766039 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS08460 (1761123) 1761123..1762427 - 1305 WP_000535012.1 restriction endonuclease subunit S -
KBW66_RS08465 (1762417) 1762417..1764036 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
KBW66_RS08470 (1764100) 1764100..1765266 - 1167 WP_000800831.1 restriction endonuclease -
KBW66_RS08475 (1765463) 1765463..1765750 + 288 WP_001295743.1 BrnT family toxin Toxin
KBW66_RS08480 (1765737) 1765737..1766039 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
KBW66_RS08485 (1766073) 1766073..1767029 - 957 WP_001295745.1 GTPase -
KBW66_RS08490 (1767040) 1767040..1767243 - 204 WP_000467859.1 YbdD/YjiX family protein -
KBW66_RS08495 (1767293) 1767293..1769443 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T199366 WP_001295743.1 NZ_CP072980:1765463-1765750 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T199366 NZ_CP097329:c3428097-3427699 [Pseudanabaena mucicola str. Chao 1806]
ATGAAATATATCCTAGATACACACTTAATTTTATGGTACTTGAGCGAAGATCCTAACCTTAGTACCAAAGCAAAAGCAAT
CGTAGATGCACGGAATGGACTGCACTTTAGTATTATCAGTCTTTGGGAAATTTACATTAAAATTAATATTGGTAAACTGC
AAATCAATCGACCTATTGAAGCATTACCAATAGAACTTCAATATATGAATATCCAAATTCTACCAATTACAATTAGGGAT
ATTGAGATATATTCCAGTCTTCCATTACCAAACACACCTATAAAACATCGAGATCCATTTGATCGCATATTAATTGCCCA
AGCAATAAACTATTCCTTCAACTTAGTAAGCAAAGATACCGCTTTTGATTCTTATCCAGTTTCACGTATATGGGATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT199366 WP_000063148.1 NZ_CP072980:1765737-1766039 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT199366 NZ_CP097329:c3428323-3428099 [Pseudanabaena mucicola str. Chao 1806]
ATGAATACCGATACCGCCTTATGGCAAGTTGTCATCAATATGCCAGACACACTCAAAACAGAACTTTTGCATTATGCAGA
ATATCTACTTAACAAATATCCAATAGCCAAAACCGTTGAGGAGCAACCTATAAAACAACAGCATGGCTATGGTAGTTGGG
CTGGTCAAATTTTTATGTCAAGTGATTTTGATGAACCATTGGAAGAACTAGAGGAATATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References