Detailed information of TA system TA199365

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1681892..1682727
• Replicon: chromosome
• Accession: NZ_CP072980
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: KBW66_RS08075
• Protein ID: WP_000854759.1
• Coordinates: 1682350..1682727 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KBW66_RS08070
• Protein ID: WP_001295723.1
• Coordinates: 1681892..1682260 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS08035 (1677598)	1677598..1677783	+	186	WP_210796687.1	hypothetical protein	-
KBW66_RS08040 (1677810)	1677810..1679423	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
KBW66_RS08045 (1679454)	1679454..1679804	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
KBW66_RS08050 (1679801)	1679801..1680226	-	426	WP_000422741.1	transposase	-
KBW66_RS08055 (1680480)	1680480..1680953	+	474	WP_001350782.1	antirestriction protein	-
KBW66_RS08060 (1680969)	1680969..1681445	+	477	WP_001186775.1	RadC family protein	-
KBW66_RS08065 (1681508)	1681508..1681729	+	222	WP_000692345.1	DUF987 domain-containing protein	-
KBW66_RS08070 (1681892)	1681892..1682260	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KBW66_RS08075 (1682350)	1682350..1682727	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
KBW66_RS08080 (1682724)	1682724..1683212	+	489	WP_000761690.1	DUF5983 family protein	-
KBW66_RS08085 (1683229)	1683229..1683405	+	177	WP_000839286.1	DUF957 domain-containing protein	-
KBW66_RS08090 (1683511)	1683511..1683660	+	150	Protein_1589	hypothetical protein	-
KBW66_RS26915 (1684027)	1684027..1684332	+	306	Protein_1590	helix-turn-helix domain-containing protein	-
KBW66_RS08100 (1684994)	1684994..1686616	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T199365 WP_000854759.1 NZ_CP072980:1682350-1682727 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T199365 NZ_CP097329:3057253-3057507 [Pseudanabaena mucicola str. Chao 1806]
GTGAAACTTATTTTTTCAGAGCGTGCTTGGGAAGATTATCTTTACTGGCAAAAAACAGACAAAAAAATATTGAATCGAAT
CAATAGTCTGATTAAAGATATTCAACGAGATCCATACAGTGGTATTGGCAAGCCAGAAGCTTTAAAACATGGATTGTCGG
GATATTGGTCTAGGCGTATCGATGATGAGCATAGGATTCTTTACAAGGTTGAAGATAATGCACTTTTATTAGTTCAGATT
CGTTATCACTATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT199365 WP_001295723.1 NZ_CP072980:1681892-1682260 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT199365 NZ_CP097329:3057005-3057256 [Pseudanabaena mucicola str. Chao 1806]
ATGAACGCAGTTTCTTATGTTGAGGCACAGAGCAACTTAGCAAAAATGATCGATCAAGTATGTGACAATCATGATCCAGT
AGTCATAACACGCCACCAACAACCATCCGCAGTGCTTCTATCACTTGAAGATTATGAATCTCTGGCAGAAACTGCTTACC
TTTTAAAAAGTCGTAATAATGCTAAGCGAATTTTTGAGGCGATCGCTGAATTAGAATCTGGCGGTGGTACTGTGCGGGAG
CTGATCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52