Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 889037..889837 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KBW66_RS04120 Protein ID WP_000342452.1
Coordinates 889310..889837 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KBW66_RS04115 Protein ID WP_001277107.1
Coordinates 889037..889303 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS04095 (884696) 884696..885364 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KBW66_RS04100 (885357) 885357..886415 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
KBW66_RS04105 (886660) 886660..887514 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KBW66_RS04110 (887785) 887785..888888 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KBW66_RS04115 (889037) 889037..889303 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KBW66_RS04120 (889310) 889310..889837 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KBW66_RS04125 (889834) 889834..890217 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KBW66_RS04130 (890640) 890640..891749 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KBW66_RS04135 (891797) 891797..892723 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KBW66_RS04140 (892720) 892720..893997 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KBW66_RS04145 (893994) 893994..894761 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T199361 WP_000342452.1 NZ_CP072980:889310-889837 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T199361 NZ_CP097329:c1001203-1000913 [Pseudanabaena mucicola str. Chao 1806]
ATGATTGAAATTCGGGAGACTAAAATCTATTCTCGGTGGTTCAAATCACTGCGCGATCGGGAAGCTAGGGCGCGTATCGA
CATTCGTATTCGGCGATTGTCTATGGGCAATCCTGGTGACGTAAAACCGATAGGAAAAGGTGTGTCAGAACTCCGTATTG
ACTATGGTGCAGGATACCGAGTGTATTTCATACAACGAGGTAATACGTTAATCATCTTGCTTGCAGGAGGCGATAAACGC
ACCCAAGATCGGGATATCAAAACAGCCTTAGACTTATCAGAAAACCTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT199361 WP_001277107.1 NZ_CP072980:889037-889303 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT199361 NZ_CP097329:c1000904-1000617 [Pseudanabaena mucicola str. Chao 1806]
ATGAACAAAGTTACAACCTCTCTTTGGGATCCAGCAGAACATCTAGAAACGAAAGAAGATATGGCAGCTTATCTCGAAGC
CGCTCTTGAAGATGGCGATCCTAATTTAGTGGTAGCAGCATTAGGAGATATTGCTCGATCTAAAGGAATGACAAATGTTG
CGCGTGAAACAGGATTAGGTCGTGAAAGCCTTTATAAATCTTTGTCAATTGACGGTAACCCAGAATTTGCAACTGTTTTA
AGAGTTATACAGTCACTTGGCATCCGTTTACGAGCTACTCCTGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References