Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 29374..29871 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KBW66_RS00160 Protein ID WP_001173343.1
Coordinates 29374..29547 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag KBW66_RS00165 Protein ID WP_001312331.1
Coordinates 29575..29871 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS00150 (26044) 26044..27501 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
KBW66_RS00155 (27738) 27738..29255 + 1518 WP_000003806.1 lysine--tRNA ligase -
KBW66_RS00160 (29374) 29374..29547 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KBW66_RS00165 (29575) 29575..29871 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KBW66_RS00170 (30099) 30099..30371 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KBW66_RS00175 (30585) 30585..34514 - 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T199359 WP_001173343.1 NZ_CP072980:c29547-29374 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T199359 NZ_CP097329:c639583-639311 [Pseudanabaena mucicola str. Chao 1806]
ATGATTAAAAATTTTAAAAATAAACTACTAGAAAGTTTGCTAAACGGTGATATGCAACCAACTACTAAAGAACACAAAAA
GGTGCAAATAAGACTTGAGCATATAGACTCAGCTTCAAAAATAGAGAATCTTAAGCTACCTAGATATGATTTACATAAAC
TAAAGGGAAATAAGAAAGGTGTTTGGTCGATCAAAACAACTGGAAATTGGCTAATTACATTTAGGTTTGAAGATGGTCAC
GCTTATGACGTAAATCACGAGGATTATCACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT199359 WP_001312331.1 NZ_CP072980:c29871-29575 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT199359 NZ_CP097329:c639220-638909 [Pseudanabaena mucicola str. Chao 1806]
ATGAACGAATTATTAGAAAGTTTAAAGAACAGAAAAACTAGACCCGTTCATCCTGGGGAGGTAATCCTAGATATTCTAGA
AGATATAGAGATTTCCCAAACTCAGTTTGCCAAAATGCTAGGAGTATCTCGCCGAACAGTTAATCAAATTATTCGGGGAC
ATAGACCAGTGACTGTGGATATGGCAATTAGAATTGGTAAAGCTTTGGGCAATGGTCCTGAATCATGGCTGAATCTTCAG
CAGAAAGTTGATCTTTGGGATGCATTACAGAAGTATGAAGAAGAATACGAGAAAGTATTGACGGTGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References