Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 119355..119998 Replicon plasmid pEC2341_2
Accession NZ_CP072977
Organism Escherichia coli strain EC2341

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KBW65_RS23580 Protein ID WP_001034044.1
Coordinates 119582..119998 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KBW65_RS23575 Protein ID WP_001261286.1
Coordinates 119355..119585 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW65_RS23555 (115986) 115986..116741 - 756 WP_000852146.1 replication initiation protein RepE -
KBW65_RS23560 (117463) 117463..118269 - 807 WP_000016970.1 site-specific integrase -
KBW65_RS23565 (118270) 118270..118575 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KBW65_RS23570 (118577) 118577..118795 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KBW65_RS23575 (119355) 119355..119585 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KBW65_RS23580 (119582) 119582..119998 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KBW65_RS23585 (120073) 120073..121638 + 1566 WP_001128474.1 AAA family ATPase -
KBW65_RS23590 (121623) 121623..122645 + 1023 WP_000361402.1 helicase UvrD -
KBW65_RS23595 (122899) 122899..123585 - 687 Protein_137 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / oqxB / oqxA / tet(B) / catA1 / fosA3 / blaCTX-M-65 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..144818 144818
- flank IS/Tn - - 122899..123246 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T199354 WP_001034044.1 NZ_CP072977:119582-119998 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T199354 NZ_CP097327:c1947106-1946903 [Providencia vermicola]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTTTCTGAAGACGAGCTGGAACTATTTTACTCAGCCGCAGATCACCGTCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATTCCAGCCTCTGTTTGGAAATTTGTTAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT199354 WP_001261286.1 NZ_CP072977:119355-119585 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT199354 NZ_CP097327:c1947553-1947185 [Providencia vermicola]
ATGGATGAATATTCACCCAAAAATTATGATATATCAGAGTTAAAATACTTGTGTAACAGTTTGAATAGAGAAGCAATTTC
AAGCTTACAGAAAACAAATACACATTGGATAAATGATTTAAGCTCACCACAAAGTGTACGGCTCAATGAGCTTATTGAAC
ATATAGCCGCCTTTGTATGGCAATATAAGATCAAACATCCAAAAGATAATCTCGTTATATCATTAGTAGAAGAGTATCTT
GACGAGACTTACGATTTATTCGGTAGTCCGGTAATAACGTTAAGTGAAATTATTGACTGGCAGAGTATGAATCAAAATCT
TGTCGCTGTACTTGATGATGATTTAAAATGCCCTGCAAGCAAGACCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References