Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 118270..118795 Replicon plasmid pEC2341_2
Accession NZ_CP072977
Organism Escherichia coli strain EC2341

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KBW65_RS23565 Protein ID WP_001159871.1
Coordinates 118270..118575 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KBW65_RS23570 Protein ID WP_000813630.1
Coordinates 118577..118795 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW65_RS23550 (114232) 114232..115398 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KBW65_RS23555 (115986) 115986..116741 - 756 WP_000852146.1 replication initiation protein RepE -
KBW65_RS23560 (117463) 117463..118269 - 807 WP_000016970.1 site-specific integrase -
KBW65_RS23565 (118270) 118270..118575 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KBW65_RS23570 (118577) 118577..118795 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KBW65_RS23575 (119355) 119355..119585 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KBW65_RS23580 (119582) 119582..119998 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KBW65_RS23585 (120073) 120073..121638 + 1566 WP_001128474.1 AAA family ATPase -
KBW65_RS23590 (121623) 121623..122645 + 1023 WP_000361402.1 helicase UvrD -
KBW65_RS23595 (122899) 122899..123585 - 687 Protein_137 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / oqxB / oqxA / tet(B) / catA1 / fosA3 / blaCTX-M-65 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..144818 144818
- flank IS/Tn - - 122899..123246 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T199353 WP_001159871.1 NZ_CP072977:c118575-118270 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T199353 NZ_CP097327:983341-983985 [Providencia vermicola]
ATGGCGTATATGAACCTAAACCCAGAAAAAGCACTTATCTGGAGAATAACCCATCGTCAGAATATTCCTTGGCTTTTTGC
CAATGGCTTATATGCAGGAAATAGTGAACATTATTCACCTAATTGGGTGACTATTGGCAATACAGACCTGATTAACCGCC
GCAATCAACGTCATGTACCTATTCAGCCGAATGGGGTACTTAATGATTATGTACCTTTCTATTTCACCCCTTTCTCGCCA
ATGATGTATAATATTTATACAGGCCGTGGTGGGGTGCGAAAAGTTCCCAATGATGATATTATCATCTTGGTTTCCAGCAT
GTATAAAATAGCCGCTCTAAAGATACCTTTTGTTTTCACCGATCGTCATGCGTATCCTGTAACATCTCAGTTCTATAACC
AGCTAACAGATCTCGCGGAGATAGATTGGGCTTTACTACAGCAGCGCAATTTTCACCGTGACCCTGATGACCCTGAAAAA
GTTGAAAGGTATCAAGCAGAAGCGTTAATTTATAAACATGTTCCTATGGAAGCCCTATTAGGTGCTGTTTGTTACACAAA
AGAAACCCAGCTAGAACTTCAAGCAAAAGCCAGCCAACTTGAGATTCATTTAGATATCCATTGCATTCCAAACTGGTACT
TTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT199353 WP_000813630.1 NZ_CP072977:c118795-118577 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT199353 NZ_CP097327:983997-985070 [Providencia vermicola]
ATGATAGAATTTACCCAAGGTAATTTGTTAGAAGCTGACGTAGAAGCGCTCGTTAATACCGTCAATACCGTTGGTGTTAT
GGGTAAAGGGATCGCATTAATGTTTAAAGAGCGTTTTCCCGCCAATATGGCTGCATATGCTAAAGCATGTAAAACACATG
AAGTGCAGACTGGAAAAATGTTTGTTACTGAAACCGGGGAGCTCATGGGCCCTCGTTGGATTGTTAATTTCCCAACAAAG
CAGCATTGGCGAGCAAAATCTCAAATTCAGTGGATTATTGATGGCTTATTGGACCTACGTAACTTCATTGAAGAGAATCA
TGTTAAATCTATTGCTATCCCGCCATTAGGGGCTGGTAATGGCGGTCTCAATTGGGATGATGTCAAGCCTCATATTGTGG
AAATATTAGGTGATATTCAAAACGTAAAAATTATTGTTTATGAGCCAATAGCAAAATATCAAAATGTTGCTAAGCCAAAG
GGTGTTGAAAAACTGACGCCGGCCCGCGCCTTAATTGCAGAACTTATCCGTCGTTATTGGGTTATGGGGATGGATTGTAG
CCTATTGGAAATACAAAAGTTAGCTTGGTTTTTAGAGCGTACAATAGAGGCTGAAGGGCTGAGAAATCCATTAAACCTAA
GGTTTGAAGCCAGAAACTATGGTCCCTACGCCGATAAGTTACGACACTTATTAAACGGGCTAGATGGAAGCTACCTCAAG
AGTGAAAAACGGATTAGTGATTGTAGTCCATTAGACGTAATTTGGTTTAATGATGATAAACGCGCTACGATTGAGCTGTA
TTTAAAAACCGAAGCAAAAGACTATTTATCCGCGCTAGAGAAGACAACGAAGCTTATTGATGGATTCGAATCTCCTTTCG
GGATAGAACTTCTCTCCACTGTAGACTGGTTGCTGTACAAAAAGCAATCTTCACCTAATATTGAGTCCTTACTTGAAGGA
ATGCGCCAATGGCCAGCAGGGGATAGATGGGCACAACGTAAACTTAGGTTATTTGACTCAAATAAGTTAGAACTTGCATT
AAATCATTTGAAACAAATACCGTTCTATAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References