Detailed information of TA system TA199348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4471948..4473536
• Replicon: chromosome
• Accession: NZ_CP072976
• Organism: Escherichia coli strain EC2341

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: KBW65_RS21535
• Protein ID: WP_001125463.1
• Coordinates: 4472214..4473536 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KBW65_RS21530
• Protein ID: WP_001296726.1
• Coordinates: 4471948..4472214 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW65_RS21530 (4471948)	4471948..4472214	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KBW65_RS21535 (4472214)	4472214..4473536	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KBW65_RS21540 (4473848)	4473848..4474056	+	209	Protein_4238	AraC family transcriptional regulator	-
KBW65_RS21545 (4474488)	4474488..4475051	+	564	WP_001373493.1	type 1 fimbrial major subunit FimA	-
KBW65_RS21550 (4475412)	4475412..4476122	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	4471948..4489372	17424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T199348 WP_001125463.1 NZ_CP072976:4472214-4473536 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T199348 NZ_CP097324:c4862715-4862341 [Citrobacter sp. XT1-2-2]
ATGGTCAAAGGGGCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTGGCGAT
AGAAACATGGCCGCCACAGAACGCAATCAGTCTCATTACATGGATGGAGGTGATGGTAGGCGCAAAAAAATACCATCAGG
AGGCCCGTACTCGCATTGCGCTAGGCGCTTTTAACATTATTGGTGTTTCACAGGATATTGCTGAGCGAAGCGTGAATATC
AGGCAAGAATACGGGATGAAATTGCCGGATGCCATTATCCTGGCTACTGCGCAGATTCATCATCTGACACTGGTTACGCG
TAATACCAAAGATTTTGCAAGCATATCCGGCGTGGTAACGCCGTATACGTTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT199348 WP_001296726.1 NZ_CP072976:4471948-4472214 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT199348 NZ_CP097324:c4862957-4862715 [Citrobacter sp. XT1-2-2]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAAACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0