Detailed information of TA system TA199339

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3931804..3932636
• Replicon: chromosome
• Accession: NZ_CP072976
• Organism: Escherichia coli strain EC2341

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: KBW65_RS18820
• Protein ID: WP_001514886.1
• Coordinates: 3931804..3932178 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: KBW65_RS18825
• Protein ID: WP_001360327.1
• Coordinates: 3932268..3932636 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW65_RS18785 (3927320)	3927320..3927793	+	474	WP_001105385.1	DNA gyrase inhibitor SbmC	-
KBW65_RS18790 (3927992)	3927992..3929050	+	1059	WP_032181141.1	FUSC family protein	-
KBW65_RS18795 (3929222)	3929222..3929551	+	330	WP_000450409.1	DUF496 family protein	-
KBW65_RS18800 (3929652)	3929652..3929918	-	267	WP_063121129.1	EutP/PduV family microcompartment system protein	-
KBW65_RS25035 (3930288)	3930288..3930659	-	372	WP_001295631.1	IS110 family transposase	-
KBW65_RS18810 (3931475)	3931475..3931555	-	81	Protein_3700	hypothetical protein	-
KBW65_RS18815 (3931655)	3931655..3931807	-	153	Protein_3701	DUF5983 family protein	-
KBW65_RS18820 (3931804)	3931804..3932178	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
KBW65_RS18825 (3932268)	3932268..3932636	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KBW65_RS18830 (3932799)	3932799..3933020	-	222	WP_000692286.1	DUF987 domain-containing protein	-
KBW65_RS18835 (3933083)	3933083..3933559	-	477	WP_001186774.1	RadC family protein	-
KBW65_RS18840 (3933575)	3933575..3934048	-	474	WP_000855059.1	antirestriction protein	-
KBW65_RS18845 (3934390)	3934390..3935208	-	819	WP_088540043.1	DUF932 domain-containing protein	-
KBW65_RS18850 (3935326)	3935326..3935521	-	196	Protein_3708	DUF905 family protein	-
KBW65_RS18855 (3935592)	3935592..3937343	-	1752	Protein_3709	Ag43/Cah family autotransporter adhesin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T199339 WP_001514886.1 NZ_CP072976:c3932178-3931804 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T199339 NZ_CP097322:c2857183-2856812 [Streptomyces sp. RerS4]
TTGACGCACTACCTGACGCTCCCCGAGCTCCTGAACCTCGCGGAGCGTCTCGGGGCGAACGAGGTGCGCGACTACGGCCT
GCTCGACTCGGCGCTCGCGCGCCCCCAGGCGAGCGTGTTCGGTCAGGACGCCTATCCGGACGTGTGGCAGAAGGCCGCGG
CACTGATGGAGTCGCTGGCCCGCAACCACGGCCTCGTGGACGGAAACAAGCGCATCGCCTGGTACGCCACGTGGGTCTTC
CTCCACATGAACGGCCATCCGCTCGACGCCGATTTCGATGTCGACGAAGCCGAACAGTTCGTGCTCGACGTATGTCAGGG
GGCGCTCGATGTCCCCAAGATCGCAGCCCAGTTGCCCCGGTTCGCGGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT199339 WP_001360327.1 NZ_CP072976:c3932636-3932268 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT199339 NZ_CP097322:c2857377-2857180 [Streptomyces sp. RerS4]
ATGGCTGGTCTCAACGTCAGATTCACCGAGGAAGAGCTGGACGCCCTGCGTGAGCGCGCCGAGGCGGAGGGGCGGAGCAT
GCAGTCCTTCGCGCACGACGCGGTCATCAAGGCCATAAACGAGCACTCCCGGCTGTTCAACGAAGCGGCGGAGCACGTGC
TCAAGGCGAGCGCCGAGCTCAATCGGAGGCTTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3

Antitoxin

• AlphaFold DB: A0A7U9IW59