Detailed information of TA system TA199337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3405367..3405992
• Replicon: chromosome
• Accession: NZ_CP072976
• Organism: Escherichia coli strain EC2341

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KBW65_RS16385
• Protein ID: WP_000911330.1
• Coordinates: 3405594..3405992 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KBW65_RS16380
• Protein ID: WP_000450524.1
• Coordinates: 3405367..3405594 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW65_RS16355 (3401170)	3401170..3401640	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KBW65_RS16360 (3401640)	3401640..3402212	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KBW65_RS16365 (3402358)	3402358..3403236	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KBW65_RS16370 (3403253)	3403253..3404287	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KBW65_RS16375 (3404500)	3404500..3405213	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KBW65_RS16380 (3405367)	3405367..3405594	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KBW65_RS16385 (3405594)	3405594..3405992	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KBW65_RS16390 (3406139)	3406139..3407002	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
KBW65_RS16395 (3407017)	3407017..3409032	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
KBW65_RS16400 (3409106)	3409106..3409804	+	699	WP_000679823.1	esterase	-
KBW65_RS16405 (3409914)	3409914..3410114	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T199337 WP_000911330.1 NZ_CP072976:3405594-3405992 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T199337 NZ_CP097322:2336996-2337271 [Streptomyces sp. RerS4]
ATGCCGATCTCGGCGAACGAGGCCCGCACCCGCTTCTACCCGTTGATCCGGCAGGTCAACGAGGATCACGAACCGATAGA
GGTCACCTCCCGCGAGCACGGCGATGTCGTCATCATGTCCGCCGAGGACTTCCGGTCCTGGCAGGAGACGGTCTACCTCC
TGCGTTCGCCCCGCAACGCGCAGATCCTCATGGAGTCCATCGCCGAGCTCGACGCCGGCCACGGGCAGGCGCGTGAACTC
GTCGAAACGACGGATGACGCGGAGTCCGCCGCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT199337 WP_000450524.1 NZ_CP072976:3405367-3405594 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT199337 NZ_CP097322:2337268-2337522 [Streptomyces sp. RerS4]
GTGAAGGTGGTGTTCTCCAGCCGTGCGTGGGACCAGTACACCTACTGGACGACGGCCGATCCGAAGATCCTCAAGAGGAT
CAATCGGCTGATCAAGGAGATTCAGCGAACACCGTTCGAAGGCGCCGGGAAGCCCGAACCGCTCAAGGAGAGCCTCTCCG
GATGGTGGTCCCGGCGCATCACGGACGAGCACCGTCTCGTCTACCGGATCAGGGACGAGGCCGTCGAAATCGCGCAGGCG
CGGTACCACTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33