Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2962343..2962997 Replicon chromosome
Accession NZ_CP072976
Organism Escherichia coli strain EC2341

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KBW65_RS14275 Protein ID WP_000244777.1
Coordinates 2962590..2962997 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KBW65_RS14270 Protein ID WP_000354046.1
Coordinates 2962343..2962609 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW65_RS14245 (2957631) 2957631..2958374 + 744 Protein_2806 SDR family oxidoreductase -
KBW65_RS14250 (2958431) 2958431..2959864 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KBW65_RS14255 (2959909) 2959909..2960220 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
KBW65_RS14260 (2960384) 2960384..2961043 + 660 WP_000250274.1 hemolysin III family protein -
KBW65_RS14265 (2961120) 2961120..2962100 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KBW65_RS14270 (2962343) 2962343..2962609 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KBW65_RS14275 (2962590) 2962590..2962997 + 408 WP_000244777.1 protein YgfX Toxin
KBW65_RS14280 (2963037) 2963037..2963558 - 522 WP_001055874.1 flavodoxin FldB -
KBW65_RS14285 (2963670) 2963670..2964566 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KBW65_RS14290 (2964591) 2964591..2965301 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KBW65_RS14295 (2965307) 2965307..2967040 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T199334 WP_000244777.1 NZ_CP072976:2962590-2962997 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199334 NZ_CP097320:3635186-3635614 [Candidatus Mycobacterium methanotrophicum]
GTGATCGTCGACTCCTCCGCGCTCATCGCCCTCATCCAGAACGAACCGGCCGCCGAGCAGGTTGCCACGGTCCTCGCCGG
CGCCCGCAGCCCGGTCATCAGCGCCGCCACCCTCACCGAAACCCTGATCGTGCTCACCGCCCGACGCGGCCCCGTCGCAC
GCACCGTCTTCGACCGCCTCAGAACCGAAATCAACCTGGGCATAGCCCAATACACCGCCGAACACGCCTACGCCGCCCAC
CGCGCCTACCTACAGTTCGGCCGCGCCCGCCATCCAGCGGGACTTAACTTCGGCGACTGCATGAGCTACGCCACCGCCGA
GCTCGCCCACGAACCGCTCCTGGCCACCGGCAACGACTTCCCGCAAACCGACCTCGAGTTCAGTGACGGCATCGTCGGCT
ACTGGCCCACGCCAAGCACTACCGACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199334 WP_000354046.1 NZ_CP072976:2962343-2962609 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199334 NZ_CP097320:3634919-3635182 [Candidatus Mycobacterium methanotrophicum]
ATGGCGCTCAACATCAAAGACCCCGAGCTGGTCCGCCTCGCCGAAGAACTCGCCGCGCGCATGGGCCACAACAACAAGAC
CAGGGCCATCCGGGACGCGCTCTCCGCCCAACTCTCGCTCCTGGAAGCCCAGCACGGCGACCGCGCCACCCATCTCCTCG
ACGTCATGCGCACCGAGATTTGGCCCCTCCTCCCGCCCCAACCGCCGATCACCAAGGACGAACGAGAGCAAATCCTGGGC
TACGACCCCGAGATCGGAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References