Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2397541..2398341 Replicon chromosome
Accession NZ_CP072976
Organism Escherichia coli strain EC2341

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KBW65_RS11440 Protein ID WP_000342449.1
Coordinates 2397814..2398341 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KBW65_RS11435 Protein ID WP_001277108.1
Coordinates 2397541..2397807 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW65_RS11415 (2393198) 2393198..2393866 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KBW65_RS11420 (2393859) 2393859..2394917 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KBW65_RS11425 (2395162) 2395162..2396016 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KBW65_RS11430 (2396288) 2396288..2397391 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KBW65_RS11435 (2397541) 2397541..2397807 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KBW65_RS11440 (2397814) 2397814..2398341 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KBW65_RS11445 (2398338) 2398338..2398721 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KBW65_RS11450 (2399145) 2399145..2400254 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KBW65_RS11455 (2400302) 2400302..2401228 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KBW65_RS11460 (2401225) 2401225..2402502 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KBW65_RS11465 (2402499) 2402499..2403266 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T199331 WP_000342449.1 NZ_CP072976:2397814-2398341 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T199331 NZ_CP097320:c2917748-2917311 [Candidatus Mycobacterium methanotrophicum]
ATGAGGATCGTCGACGCCAATGTCCTGTTGTACGCCGTCAACACCGCAGCAGGCCACCACGAGGCCTCCCGCCGCTGGCT
CGACGGTGCGCTGTCCGGCAGAGACACCGTCGGCCTGGCGTGGGTGCCGCTGCTCGCATTTGTGCAATTGACGACTCGTC
ATGGGCTTTTTCCTTCGCCCCTGACCGCCGACGCGGCAATGGCTCAAGTGCTCGATTGGTGCAACGCACCAGCGTCCGTC
ATCGTGTCGCCGACTGCGCGCCATGGCGACGTGCTGTCCGGGCTGCTGTCGCAGGTTGGCGCGGGCGGAAACCTCGTCAA
CGATGCGCATCTGGCCGCTTTGGCGATTGAGCACCGCGCACGAATAGTTAGTTACGACAGCGATTTCGGACGATTCGATG
GTGTGCGGTGGGAAACGCCCGGGTCATTGTCGAACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT199331 WP_001277108.1 NZ_CP072976:2397541-2397807 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT199331 NZ_CP097320:c2917999-2917745 [Candidatus Mycobacterium methanotrophicum]
GTGCGGACGACGGTGACCCTTGACGACGACACGCTCGTCGTCGTCAGGCAGCTGATGCGTCAGCGCAACGTATCGTTCAA
GCAGGCGCTCAATGACGCCATCCGGCAAGGTGCGCAACGGCGGCCGGCTGGTGCGGTTTTCCAAACGCGGACAGCTGATT
TGGGTGTGCCCTCGGTCAACCTGGACCGGGCGCTGCAGATCGCCGGCGAACTCGAGGACGAGGACCTGATCCGGCGACAG
CGTCGCGGCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References