Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1559444..1559941 Replicon chromosome
Accession NZ_CP072976
Organism Escherichia coli strain EC2341

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KBW65_RS07605 Protein ID WP_001173343.1
Coordinates 1559444..1559617 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag KBW65_RS07610 Protein ID WP_001425728.1
Coordinates 1559645..1559941 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW65_RS07595 (1556114) 1556114..1557571 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KBW65_RS07600 (1557808) 1557808..1559325 + 1518 WP_001295074.1 lysine--tRNA ligase -
KBW65_RS07605 (1559444) 1559444..1559617 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KBW65_RS07610 (1559645) 1559645..1559941 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KBW65_RS07615 (1560168) 1560168..1560440 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KBW65_RS07620 (1560452) 1560452..1560682 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KBW65_RS07625 (1560863) 1560863..1562494 + 1632 WP_001216487.1 sensor histidine kinase -
KBW65_RS07630 (1562491) 1562491..1563210 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T199329 WP_001173343.1 NZ_CP072976:c1559617-1559444 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T199329 NZ_CP097320:2603519-2603938 [Candidatus Mycobacterium methanotrophicum]
GTGAAGTTCGCCGACACGGGTTGGTGGGTGGCGTGGGCGCTGCCCGGGGATTCTCGGCACAATCACGCGCTGGGCATGCT
GGGTGCTCTCGGACGGTCCGAGCAGATCCTGACGACTAACCTTGTTGCGGGCGAGACCTGGACGTTCCTGCGGCGCAAGG
ACAGCCACCGGACTGCGCTGGCCTTTCTCGATCGGCTCGACACTCTGAGGAATGCCGAGCGCCTCGTCGTGCACCGAGTT
ACAGAGGGCCAAGAAGCGGCAGCGTGGGACTGGCTTCGCAAACACGACGAGCGGGTGTACTCGTACGTGGACGCAACGAG
TTTTCGTGTCATGCGGGACCGGCGGCTGCGCGAAGCCCTAGCGTTCGACCAGGACTTCGCGGCCACCGGATTCGTAGAGG
TCCGGGCCAACCCGTGGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT199329 WP_001425728.1 NZ_CP072976:c1559941-1559645 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT199329 NZ_CP097320:2604080-2604328 [Candidatus Mycobacterium methanotrophicum]
ATGAAAACAGCTATCTCCCTCTCGGACGAGACGTTCGATCGCGTCTCTCGGACGGCGAGTGACCTCGGCATGAGTCGATC
CGAGTTCTTCGCCCGAGCTGCGCAGCGTTACTTGGACGAGTTAGACGCCCAGTCGCTGACCGGTCAGATCGACAGTGCTT
TGGAGCGTGTCGATGGGACCGACGAGGCCAAGGATGCTGCCGTGGCTATCGGGCACCGCGTGTTGGACGCCGTGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References