Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 42141..42938 Replicon plasmid unnamed2
Accession NZ_CP072972
Organism Enterobacter sp. BWH 37

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag IBG18_RS25385 Protein ID WP_006812498.1
Coordinates 42414..42938 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag IBG18_RS25380 Protein ID WP_006812497.1
Coordinates 42141..42410 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBG18_RS25355 37521..38861 - 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -
IBG18_RS25360 38922..39647 - 726 WP_006812583.1 hypothetical protein -
IBG18_RS25365 39911..40708 + 798 WP_006812584.1 DUF2971 domain-containing protein -
IBG18_RS25370 40750..41103 - 354 WP_160859104.1 hypothetical protein -
IBG18_RS25375 41109..41774 - 666 WP_023315962.1 AAA family ATPase -
IBG18_RS25380 42141..42410 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
IBG18_RS25385 42414..42938 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
IBG18_RS25390 42965..43129 - 165 WP_006812499.1 hypothetical protein -
IBG18_RS25395 43122..43373 - 252 WP_000147960.1 hypothetical protein -
IBG18_RS25400 43375..44067 - 693 WP_006812500.1 membrane protein -
IBG18_RS25405 44081..44404 - 324 WP_000064173.1 hypothetical protein -
IBG18_RS25410 44479..45267 - 789 WP_032655902.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..116629 116629


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T199327 WP_006812498.1 NZ_CP072972:42414-42938 [Enterobacter sp. BWH 37]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T199327 NZ_CP097320:2551280-2551591 [Candidatus Mycobacterium methanotrophicum]
ATGCGACCCATCCACATCGCCCAGCTTGACAAAGCTAGACCCGTCCTGATTCTCACCCGCGAGGTCGTGCGTCCACACCT
CACCAACGTCACCGTCGGCCCAATCACGACGACCGTCCGTGGGCTGGCCACCGAAGTCCCGGTAGGCATCGTGAACGGGC
TGAATCAACCGTCGGTGGTCAGTTGCGACAACATCCAAACGATCCCGGTGTCTGACCTTGGTCGTCAAATCGGCTACCTT
CTCGCGTCGCAGGAGCCAGCCCTAGCAGACGCCATCGGCAACGCGTTCGACCTCGATTGGGTCGTGTCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT199327 WP_006812497.1 NZ_CP072972:42141-42410 [Enterobacter sp. BWH 37]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT199327 NZ_CP097320:2551035-2551280 [Candidatus Mycobacterium methanotrophicum]
ATCTATGAAGTGAGCACACAAATTGCGGTCCGCTTACCCGACGAGATCGTCGCTTTCATCGATGACGAAGTCCGGGGACA
ACATGCCCGCAGCCGTGCGGCTGTCGTGGTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGG
AGATCCTCGCGGCCAGCTTGTCAGCGCCGAGTGATTTGGATGCGCTGGCCGGCCGCTCTGCTCGTACCGTCCTCGACATC
GACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References