Detailed information of TA system TA199324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4585585..4586192
• Replicon: chromosome
• Accession: NZ_CP072970
• Organism: Enterobacter sp. BWH 37

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: IBG18_RS22450
• Protein ID: WP_057979982.1
• Coordinates: 4586007..4586192 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IBG18_RS22445
• Protein ID: WP_023315016.1
• Coordinates: 4585585..4585992 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG18_RS22430	4582328..4583581	+	1254	WP_015572749.1	valine--pyruvate transaminase	-
IBG18_RS22435	4583589..4584044	-	456	WP_023323869.1	4Fe-4S dicluster domain-containing protein	-
IBG18_RS22440	4584505..4585581	+	1077	WP_023323870.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
IBG18_RS22445	4585585..4585992	-	408	WP_023315016.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IBG18_RS22450	4586007..4586192	-	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
IBG18_RS22455	4586442..4587980	-	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
IBG18_RS22460	4588147..4589031	+	885	WP_003860164.1	ROK family protein	-
IBG18_RS22465	4589035..4590873	-	1839	WP_023323871.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T199324 WP_057979982.1 NZ_CP072970:c4586192-4586007 [Enterobacter sp. BWH 37]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T199324 NZ_CP097320:c1256688-1256422 [Candidatus Mycobacterium methanotrophicum]
GTGAGTTGGACAGTGGGCCTTGCCAGCAGTGCGCGCCGCGACATCGACAAGCTGCCGCCGAGGGTAATACCCGCTGTGGT
GGAGTTCATCTATGGCCCTCTTGCTAGTGATCCGCGCCGCATCGGCAAGCCGCTGCGCGAAAATTTTCACGGACACTGGT
CTGCGCGCCGCGGCGACTACCGTGTGCTGTACATGCTTGATGAGCACCGTCAGCAGATCGTGATCATTAGAGTCGGCCAC
CGCTCCCAAGTCTACAGGCCCGGATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15205.20 Da
• Isoelectric Point: 4.5036

>AT199324 WP_023315016.1 NZ_CP072970:c4585992-4585585 [Enterobacter sp. BWH 37]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT199324 NZ_CP097320:c1256960-1256685 [Candidatus Mycobacterium methanotrophicum]
ATGACTACCTTGCCGCTGACCGAAGCAAAGGCGAAGTTGAACGAGTTGATCGACAGTGCCATCAACACCCACGAACGCGT
GACCATTACCAGGCACGGCAAGCCCGCGGCGGTACTGATGTCTGTTGAGGATCTAGAGGCTCTTGAAGAGACGCTCTACT
GGCAGTCGCAGCCCGGCGTACATGAGGACATCGCGAAGGCGCGCGAGCAAGCCGAGGCGGGTTTGCTGATGACCGAGGGC
GAGGTCCGGCGCCGTTATGGCGTTGGCCACCGGTGA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0