Detailed information of TA system TA199312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 301624..302220
• Replicon: chromosome
• Accession: NZ_CP072970
• Organism: Enterobacter sp. BWH 37

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: IBG18_RS01445
• Protein ID: WP_023315213.1
• Coordinates: 301918..302220 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IBG18_RS01440
• Protein ID: WP_032610152.1
• Coordinates: 301624..301911 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG18_RS01435	299996..301627	+	1632	WP_032610151.1	Na/Pi cotransporter family protein	-
IBG18_RS01440	301624..301911	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
IBG18_RS01445	301918..302220	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IBG18_RS01450	302418..303290	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
IBG18_RS01455	303291..303563	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
IBG18_RS01460	303614..304558	-	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
IBG18_RS01465	304652..306001	-	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T199312 WP_023315213.1 NZ_CP072970:c302220-301918 [Enterobacter sp. BWH 37]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T199312 NZ_CP097294:171529-171795 [Vibrio sp. J383]
ATGACGAATGATCGAAAGATTGTATTTACAGATAATGCTTGGGAAGATTACCTATACTGGCAAACCCAAGACAAGAAAAC
GCTCAAGAAACTAAATAAGTTGATTGAAGCTGCCAAAAGAACGCCCTTTGAAGGGATTGGAAAGCCGGAGCCACTAGTCG
GCAACCTGTCTGGATTTTGGTCAAGACGAATCGATGACAAGAATCGATTAGTGTATAAAGCAGATGACGATAACTTTTAC
ATAATTGCTTGTCGTCACCACTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT199312 WP_032610152.1 NZ_CP072970:c301911-301624 [Enterobacter sp. BWH 37]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT199312 NZ_CP097294:171282-171539 [Vibrio sp. J383]
ATGAATATTGTTTCTTTTACTGAGGCAAGAAATAGCCTTAAGTCTGTGCTCGATAGAGTGGTAGACGATTCTGATGTTAC
GGTAATAACCAGACGAGATTCAGAAGATGCCGTGGTAATGTCTCTTGATCATTTTAATTCAATGCAAGAAACCTTGTACC
TAATGAGCTCGCCTAAAAACGCTGAAAGATTGGCTGAATCCATTGCTCAACTCGAAGCCGGAAATACAACCGTTCGAGAA
TTGAATAATGACGAATGA

Structures

Toxin

• AlphaFold DB: A0A837F9W8