Detailed information of TA system TA199309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 342718..343619
• Replicon: plasmid unnamed1
• Accession: NZ_CP072962
• Organism: Enterobacter sp. MGH 14

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A330G4J7
• Locus tag: IBG17_RS25065
• Protein ID: WP_022652120.1
• Coordinates: 342718..343176 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IBG17_RS25070
• Protein ID: WP_022652121.1
• Coordinates: 343173..343619 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG17_RS25030	338038..338673	+	636	WP_012477419.1	hypothetical protein	-
IBG17_RS25035	338701..339756	-	1056	WP_022652116.1	phosphoadenosine phosphosulfate reductase family protein	-
IBG17_RS25040	339934..340380	-	447	WP_015063027.1	hypothetical protein	-
IBG17_RS25045	340427..341233	-	807	WP_015063028.1	hypothetical protein	-
IBG17_RS25050	341463..341726	-	264	WP_032610382.1	hypothetical protein	-
IBG17_RS25055	341783..342115	-	333	WP_022652118.1	hypothetical protein	-
IBG17_RS25060	342450..342680	-	231	WP_022652119.1	hypothetical protein	-
IBG17_RS25065	342718..343176	-	459	WP_022652120.1	RES family NAD+ phosphorylase	Toxin
IBG17_RS25070	343173..343619	-	447	WP_022652121.1	DUF2384 domain-containing protein	Antitoxin
IBG17_RS25075	343696..344004	-	309	WP_022652122.1	hypothetical protein	-
IBG17_RS25080	344180..345448	-	1269	WP_022652123.1	DNA methyltransferase	-
IBG17_RS25085	345435..346046	-	612	WP_022652124.1	hypothetical protein	-
IBG17_RS25090	346108..346743	-	636	WP_022652125.1	hypothetical protein	-
IBG17_RS25095	346859..347116	-	258	WP_022652126.1	hypothetical protein	-
IBG17_RS25100	347180..347614	-	435	WP_015063073.1	hypothetical protein	-
IBG17_RS25105	347750..348127	-	378	WP_015063074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-30 / blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / blaTEM-1B / aadA2 / qacE / sul1 / qnrB2 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia	-	1..423968	423968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16758.14 Da
• Isoelectric Point: 4.4843

>T199309 WP_022652120.1 NZ_CP072962:c343176-342718 [Enterobacter sp. MGH 14]
VILYRMTKTRYITTAWSGLGAKEAGGRWNSVGTALVYLSETASLTMLETLVHINAPQLLDAYTLLSIDVPDDQIQAFDLN
LLPPGWASEDAPAELALYGDNWAESGSSVALRIPSALSPVEFNYLLNPAHPAIFDLMKTVQKIPYQYDTRLK

Nucleotide

• Length: 459 bp

>T199309 NZ_CP097291:3826723-3826983 [Providencia sp. PROV188]
ATGAGTAGACTTCTAACATGGACGGATCAAGCTTGGGAAGATTATCTATACTGGCAAAGCCAAGATAAAAAGACATTAAA
GCGGATCAATAAACTCATTGAAGACACAAAAAGAACGCCATTCAGCGGTATAGGTAAACCAGAGCCACTAAAAGAAAACT
TAGCTGGCTTCTGGTCTCGTAGAATAGATGAAACTAATAGGCTAGTTTATGCAATCGAAAATACCGCACTAATCATCATT
GCCTGTCGCTATCATTACTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16606.03 Da
• Isoelectric Point: 7.4048

>AT199309 WP_022652121.1 NZ_CP072962:c343619-343173 [Enterobacter sp. MGH 14]
MTMKVFSPDLSKMNPHGLWQVVGLDNDGIALMDKISHGLEGKVAHSISEWANITPSELRKMSGIPNTTFNRSVKARFTAD
QSERLVRIIRVIERAVELFEGDKEAAQKWMNEPNRGLSWKTPADVVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT199309 NZ_CP097291:3826476-3826730 [Providencia sp. PROV188]
ATGAAAACCATTTCCTTTAGTGATGCTAGAAGCAATTTGAAAGCAGTGCTTGATAGAGTTGTTGATGATATGGATACGAC
GATTATAACCCGCCGTGATTCTGAAGCGGCTGTAGTTATGTCCTTGGAATACTACAATAGTTTAATGGAAACCATCTACC
TATTGCGCTCTCCTGCAAATGCAGAGCATCTCAACAAATCCATCGAGCAATTTAAAGCGGGTAAGATTTCAGAGAGAAAG
CTAATCAATGAGTAG

Structures

Toxin

• AlphaFold DB: A0A330G4J7