Detailed information of TA system TA199306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3132758..3134541
• Replicon: chromosome
• Accession: NZ_CP072961
• Organism: Enterobacter sp. MGH 14

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A8B5ZXW9
• Locus tag: IBG17_RS15220
• Protein ID: WP_022651516.1
• Coordinates: 3132758..3134107 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: IBG17_RS15225
• Protein ID: WP_003859317.1
• Coordinates: 3134104..3134541 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG17_RS15195	3128581..3129516	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
IBG17_RS15200	3129685..3130080	+	396	WP_003859308.1	CidA/LrgA family protein	-
IBG17_RS15205	3130077..3130772	+	696	WP_003859310.1	CidB/LrgB family autolysis modulator	-
IBG17_RS15210	3130901..3131785	+	885	WP_022651515.1	cytidine deaminase	-
IBG17_RS15215	3131913..3132623	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
IBG17_RS15220	3132758..3134107	+	1350	WP_022651516.1	HipA domain-containing protein	Toxin
IBG17_RS15225	3134104..3134541	+	438	WP_003859317.1	helix-turn-helix transcriptional regulator	Antitoxin
IBG17_RS15230	3134580..3135590	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
IBG17_RS15235	3135606..3137126	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
IBG17_RS15240	3137206..3138204	-	999	WP_003859322.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
IBG17_RS15245	3138500..3139522	-	1023	WP_003859324.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51151.85 Da
• Isoelectric Point: 4.7049

>T199306 WP_022651516.1 NZ_CP072961:3132758-3134107 [Enterobacter sp. MGH 14]
METLTVQAYLNETWTDIALIKYPGSEKKDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T199306 NZ_CP097291:c3798339-3798052 [Providencia sp. PROV188]
ATGAATATTAAAATAAAAGAGTATGACATTGCTGAGCACTTAACGAACGAAGAAGAAATTCAGCTGTATCTTAATGAAAT
ATTAGAAGAAGGCGATACGGCACTCATTCTTGCGGCATTAGGCGATATTGCGAGAGCGAGAAATATGACCCAATTAGCAA
AGGAAGTAGGAATGAGCCGAGAAGGGCTATATAAGGCACTATCTGGTGAAGGTAACCCTGCATTTACCACTATCTTGAAG
ATAGTTCATTCACTAGGACTAAAGTTGCAATTTAAGAGCGCAAGATAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT199306 WP_003859317.1 NZ_CP072961:3134104-3134541 [Enterobacter sp. MGH 14]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT199306 NZ_CP097291:c3798599-3798393 [Providencia sp. PROV188]
ATGCATGACCTGAAAGATATTCGAGCAAAAACAAAAATACAGATAAGAATTCGGCGTTTGAAGCAAGGCAATTTTGGTGA
TGTTGAATCAATAGGTGATGGTTTTTCTGAATTAAAAATACATGAAGGGAAAGGATATCGCGTTTATTTAAGAAAGATTG
ATAATACAATCGTATTGTTAATCAGCGGTGGTGATAAATCGACGTAG

Structures

Toxin

• AlphaFold DB: A0A8B5ZXW9

Antitoxin

• AlphaFold DB: A0A837FFV9