Detailed information of TA system TA199294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 594913..595616
• Replicon: chromosome
• Accession: NZ_CP072961
• Organism: Enterobacter sp. MGH 14

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A4V2LVF0
• Locus tag: IBG17_RS02750
• Protein ID: WP_022650317.1
• Coordinates: 595275..595616 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: IBG17_RS02745
• Protein ID: WP_022650316.1
• Coordinates: 594913..595254 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG17_RS02710	589999..590478	+	480	WP_123971091.1	hypothetical protein	-
IBG17_RS02715	590710..591585	+	876	WP_022650310.1	GTPase family protein	-
IBG17_RS02720	591895..592359	+	465	WP_022650311.1	hypothetical protein	-
IBG17_RS02725	592460..592933	+	474	WP_022650312.1	hypothetical protein	-
IBG17_RS02730	593052..593873	+	822	WP_022650313.1	DUF932 domain-containing protein	-
IBG17_RS02735	593904..594347	+	444	WP_022650314.1	antirestriction protein	-
IBG17_RS02740	594360..594902	+	543	WP_022650315.1	DNA repair protein RadC	-
IBG17_RS02745	594913..595254	+	342	WP_022650316.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IBG17_RS02750	595275..595616	+	342	WP_022650317.1	TA system toxin CbtA family protein	Toxin
IBG17_RS02755	595867..596874	+	1008	WP_022650318.1	restriction endonuclease	-
IBG17_RS02760	597055..597891	-	837	Protein_531	HNH endonuclease	-
IBG17_RS02765	598191..598658	+	468	WP_022650322.1	SymE family type I addiction module toxin	-
IBG17_RS02770	598716..599087	-	372	WP_022650323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	572237..603558	31321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12795.68 Da
• Isoelectric Point: 6.0705

>T199294 WP_022650317.1 NZ_CP072961:595275-595616 [Enterobacter sp. MGH 14]
MKTLPATNPQAAKLCLSPVAVWQMLLALLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T199294 NZ_CP097286:5675695-5676021 [Pseudomonas viridiflava]
GTGAAGCAGCACGGATTCAATCGCGCAGATATCGTCCGCATCAGCCTCAATCCAACCGCTGGCCGAGAACAGCAGCGCGA
TTTTCGTCCCGCGTTGGTGCTGACACCAGCAGCGTATAACGTCTCCGGCCTTGCGATCGTTGCGCCTATCATACAGGGCG
GTGATTTTGCGCGTTATTCGGGGTTCGCTGTTCCTTTGGGTGGGTCGGGAGTCCCAAACAAAAGCCCCCGGAGACTCACG
TCGCCGGGGGCTTTTGATGACCGCAGGAGAATTACAGGCCGGCAGCAGCGCGCAGGGCGTCGGCGCGGTCGGTTTTTTCC
CAGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12670.27 Da
• Isoelectric Point: 6.8219

>AT199294 WP_022650316.1 NZ_CP072961:594913-595254 [Enterobacter sp. MGH 14]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYPADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLHHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT199294 NZ_CP097286:5675444-5675698 [Pseudomonas viridiflava]
ATGGAAGTCAAAATTCAGCAGTGGGGCAATAGCGCAGCAATCAGATTGCCAGCCAATGTGCTCAAGCAGATGAGCCTGGT
CAGCGGCGATGTGCTGGTGCTGGATGTTTCCAGCGAAGTGATGACGCTCAGGCCTGCCAAGGCCAGACTGCATTACCAAT
TGGCCGACCTTATGGCCCAATGTGATTTGAATGCGTCCGAGCCAGAGGAGTTGGCTGGTTGGAACGCCATGCAACCCATA
GGGCGTGAAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A4V2LVF0