Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 36030..36556 Replicon plasmid unnamed1
Accession NZ_CP072954
Organism Enterobacter sp. MGH 3

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IBG19_RS24145 Protein ID WP_000323025.1
Coordinates 36269..36556 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q5J3S4
Locus tag IBG19_RS24140 Protein ID WP_000534857.1
Coordinates 36030..36269 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBG19_RS24130 33886..34491 + 606 WP_000509966.1 recombinase family protein -
IBG19_RS24135 35033..35773 - 741 WP_022652289.1 hypothetical protein -
IBG19_RS25620 35895..36005 - 111 Protein_32 hypothetical protein -
IBG19_RS24140 36030..36269 + 240 WP_000534857.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IBG19_RS24145 36269..36556 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IBG19_RS24150 36839..37063 + 225 Protein_35 hypothetical protein -
IBG19_RS24155 37394..38374 + 981 WP_022652288.1 IS5-like element IS5 family transposase -
IBG19_RS24160 38533..38709 + 177 WP_022652287.1 hypothetical protein -
IBG19_RS24165 38888..39520 + 633 WP_022652286.1 recombinase family protein -
IBG19_RS24170 39574..39774 + 201 WP_022652285.1 hypothetical protein -
IBG19_RS24175 39921..40871 + 951 WP_022652284.1 Rpn family recombination-promoting nuclease/putative transposase -
IBG19_RS24180 40868..41479 - 612 WP_022652283.1 DUF2913 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(D) / sul1 / qacE / ere(A) / aac(6')-IIc / sul2 / blaTEM-1A / aph(3')-Ia - 1..241263 241263
- inside IScluster/Tn tet(D) / sul1 / qacE / ere(A) / aac(6')-IIc - 30182..72723 42541


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T199291 WP_000323025.1 NZ_CP072954:36269-36556 [Enterobacter sp. MGH 3]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T199291 NZ_CP097269:3158376-3158720 [Acidovorax sp. GBBC 1281]
ATGTTTATCCCGAATCGTGGTGACATCGTGCACCTTGAATTTGACCCCGCATCAGGTAAAGAGATGAAGGGCAAGCACTA
CGCCTTGGTGCTCAGCTCTAAAGAATTCAATCGTCGAGGACTGGCGATGGTTTGCCCGATTAGTCAGGGAGCAGCTGAGT
CGGCTAGGACCCACGGGACTTTGGTCTCATTGATGAATACAGGCACGGATACGCAGGGAACTGTGCATTGTCATCAGTTG
AAATCGCTAGATTGGAAGGAGCGTCGCTTCAAACATAAAGAGACTGTTCCTGACGAGGTTATGGATGAGGTCAATGCCCG
AGTGAGTGCAATTTTGTTTGATTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9085.55 Da        Isoelectric Point: 4.6820

>AT199291 WP_000534857.1 NZ_CP072954:36030-36269 [Enterobacter sp. MGH 3]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL

Download         Length: 240 bp

>AT199291 NZ_CP097269:3158134-3158376 [Acidovorax sp. GBBC 1281]
ATGAAATTAAGCATTCAGAAATGGGGCAATAGCGCGGCGCTTCGTCTACCGGTCGGTATGCTCTCCGCACTTGGGGCAAA
GGTTGGGGATGCGTTTGAGGTCGATTTATCTGCCGGCCAAGTGCTCTTGCGAGTGGCCAAGCCACGTTATAAATTGGCTG
ATCTACTTGCAGAGATGCCAGAGCAACTTCCCCGTGTGGAAGGCTGGGACGCGATGACAGCGGTAGGTCTTGAGGTGATG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE60

References