Detailed information of TA system TA199275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 72404..73097
• Replicon: plasmid unnamed2
• Accession: NZ_CP072951
• Organism: Klebsiella pneumoniae MGH 39

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: IBD60_RS27765
• Protein ID: WP_000182276.1
• Coordinates: 72741..73097 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: IBD60_RS27760
• Protein ID: WP_001172026.1
• Coordinates: 72404..72739 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBD60_RS28265	67511..67671	+	161	Protein_80	protein CopA/IncA	-
IBD60_RS27730	67638..67715	+	78	WP_011201829.1	RepA leader peptide Tap	-
IBD60_RS27735	67696..68571	+	876	WP_024191793.1	incFII family plasmid replication initiator RepA	-
IBD60_RS27740	69929..71209	-	1281	WP_064734190.1	HD-GYP domain-containing protein	-
IBD60_RS27745	71327..71707	-	381	WP_001054412.1	hypothetical protein	-
IBD60_RS27750	71704..72030	-	327	WP_000091614.1	hypothetical protein	-
IBD60_RS27755	72054..72389	-	336	WP_000741275.1	hypothetical protein	-
IBD60_RS27760	72404..72739	-	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
IBD60_RS27765	72741..73097	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IBD60_RS27770	73289..73891	+	603	WP_010465829.1	recombinase family protein	-
IBD60_RS27775	73875..76904	+	3030	WP_032432545.1	Tn3 family transposase	-
IBD60_RS27780	77141..77986	+	846	WP_003464995.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-3 / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib	-	1..120604	120604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T199275 WP_000182276.1 NZ_CP072951:c73097-72741 [Klebsiella pneumoniae MGH 39]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T199275 NZ_CP097262:3411985-3412203 [Salmonella enterica subsp. enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT199275 WP_001172026.1 NZ_CP072951:c72739-72404 [Klebsiella pneumoniae MGH 39]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT199275 NZ_CP097262:3411583-3411957 [Salmonella enterica subsp. enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7