Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 59041..59777 Replicon plasmid unnamed1
Accession NZ_CP072950
Organism Klebsiella pneumoniae MGH 39

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag IBD60_RS26405 Protein ID WP_003026803.1
Coordinates 59295..59777 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag IBD60_RS26400 Protein ID WP_003026799.1
Coordinates 59041..59307 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBD60_RS26385 (56118) 56118..56573 - 456 Protein_53 transposase domain-containing protein -
IBD60_RS28210 (58101) 58101..58406 + 306 WP_227522558.1 CPBP family intramembrane metalloprotease -
IBD60_RS26395 (58565) 58565..58817 - 253 Protein_55 transposase -
IBD60_RS26400 (59041) 59041..59307 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
IBD60_RS26405 (59295) 59295..59777 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
IBD60_RS26410 (59978) 59978..61381 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
IBD60_RS26415 (61410) 61410..62042 - 633 WP_001567369.1 hypothetical protein -
IBD60_RS26420 (62219) 62219..63384 - 1166 Protein_60 IS3 family transposase -
IBD60_RS26425 (63561) 63561..64523 - 963 WP_004152113.1 zinc metalloprotease HtpX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..235674 235674
- inside IScluster/Tn - - 58562..63246 4684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T199274 WP_003026803.1 NZ_CP072950:59295-59777 [Klebsiella pneumoniae MGH 39]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T199274 NZ_CP097262:c2693479-2693376 [Salmonella enterica subsp. enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT199274 WP_003026799.1 NZ_CP072950:59041-59307 [Klebsiella pneumoniae MGH 39]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT199274 NZ_CP097262:2693374-2693519 [Salmonella enterica subsp. enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References