Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4691067..4691877 Replicon chromosome
Accession NZ_CP072949
Organism Klebsiella pneumoniae MGH 39

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IBD60_RS22890 Protein ID WP_002887280.1
Coordinates 4691067..4691600 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IBD60_RS22895 Protein ID WP_002887278.1
Coordinates 4691611..4691877 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBD60_RS22885 4689899..4691020 + 1122 WP_009309849.1 cupin domain-containing protein -
IBD60_RS22890 4691067..4691600 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
IBD60_RS22895 4691611..4691877 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
IBD60_RS22900 4691980..4693413 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
IBD60_RS22905 4693403..4694086 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
IBD60_RS22910 4694259..4695644 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
IBD60_RS22915 4695662..4695967 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T199269 WP_002887280.1 NZ_CP072949:c4691600-4691067 [Klebsiella pneumoniae MGH 39]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T199269 NZ_CP097262:358943-359218 [Salmonella enterica subsp. enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT199269 WP_002887278.1 NZ_CP072949:c4691877-4691611 [Klebsiella pneumoniae MGH 39]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT199269 NZ_CP097262:358680-358940 [Salmonella enterica subsp. enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References