Detailed information of TA system TA199258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1837051..1837641
• Replicon: chromosome
• Accession: NZ_CP072949
• Organism: Klebsiella pneumoniae MGH 39

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: IBD60_RS08910
• Protein ID: WP_023341911.1
• Coordinates: 1837309..1837641 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: IBD60_RS08905
• Protein ID: WP_000288812.1
• Coordinates: 1837051..1837308 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBD60_RS08880	1833651..1835000	-	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-
IBD60_RS08885	1835008..1835424	-	417	WP_124047552.1	hypothetical protein	-
IBD60_RS08895	1836052..1836513	+	462	WP_032431987.1	hypothetical protein	-
IBD60_RS08900	1836510..1836716	+	207	WP_022615589.1	helix-turn-helix transcriptional regulator	-
IBD60_RS08905	1837051..1837308	+	258	WP_000288812.1	antitoxin	Antitoxin
IBD60_RS08910	1837309..1837641	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IBD60_RS08920	1837963..1839399	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
IBD60_RS08930	1839765..1841219	-	1455	WP_004148975.1	AMP nucleosidase	-
IBD60_RS08935	1841349..1841594	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T199258 WP_023341911.1 NZ_CP072949:1837309-1837641 [Klebsiella pneumoniae MGH 39]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T199258 NZ_CP097256:c141139-140858 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT199258 WP_000288812.1 NZ_CP072949:1837051-1837308 [Klebsiella pneumoniae MGH 39]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT199258 NZ_CP097256:c141363-141136 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0