Detailed information of TA system TA199257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1489340..1490076
• Replicon: chromosome
• Accession: NZ_CP072949
• Organism: Klebsiella pneumoniae MGH 39

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IBD60_RS07365
• Protein ID: WP_032432065.1
• Coordinates: 1489594..1490076 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IBD60_RS07360
• Protein ID: WP_003026799.1
• Coordinates: 1489340..1489606 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBD60_RS07320	1485184..1485546	-	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-
IBD60_RS07325	1485598..1485948	-	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
IBD60_RS07330	1486306..1486554	+	249	WP_004193994.1	hypothetical protein	-
IBD60_RS07335	1486551..1487762	+	1212	WP_032415728.1	hypothetical protein	-
IBD60_RS07340	1487896..1488387	+	492	WP_004206662.1	hypothetical protein	-
IBD60_RS07345	1488448..1488651	+	204	WP_032432068.1	HHA domain-containing protein	-
IBD60_RS07350	1488665..1488895	+	231	WP_032432067.1	hypothetical protein	-
IBD60_RS07355	1488939..1489133	-	195	WP_032432066.1	hypothetical protein	-
IBD60_RS07360	1489340..1489606	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IBD60_RS07365	1489594..1490076	+	483	WP_032432065.1	GNAT family N-acetyltransferase	Toxin
IBD60_RS07370	1490438..1491037	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
IBD60_RS07375	1491249..1492193	-	945	WP_077254249.1	fimbrial protein	-
IBD60_RS07380	1492205..1492783	-	579	WP_032432061.1	type 1 fimbrial protein	-
IBD60_RS07385	1492787..1493527	-	741	WP_048978466.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1489340..1501018	11678

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17268.93 Da
• Isoelectric Point: 8.7400

>T199257 WP_032432065.1 NZ_CP072949:1489594-1490076 [Klebsiella pneumoniae MGH 39]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T199257 NZ_CP097255:c4539273-4538962 [Enterobacter bugandensis]
ATGCTATTCATTGAAACGGACATTTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATCGGTTCCAGAT
ATTCCTGGCGACACAACCGGAGTACGGCGATGTTATCCAGAATACTGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GAAAGGGAAAGCGTGGTGGAGTCAGAGTTATCTATTTTTACCGTACGTGTGAGTTTGAAATCAGACTGCTGCTCATCTAT
CGCAAAGGGATTAAAGATGACCTGTCTGCGGGGGAGAAAGCTATTCTGAAAAAGATGATAGAGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT199257 WP_003026799.1 NZ_CP072949:1489340-1489606 [Klebsiella pneumoniae MGH 39]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT199257 NZ_CP097255:c4538961-4538671 [Enterobacter bugandensis]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCGTTGGGCAAATGAACGAGATTGCCGAGGGCCAACGCGAGCCGTC
GCGTATCTTTCAAATCGATGCGATGAGAATAAAAGAAATTCGTCAGGCATCCGGGTTGTCGCAGAGTAAATTCGCGACCT
TAATCTCTGTCAGCGTTGATACATTGCGTAACTGGGAACAAGGCAGACGCTCGCCTACAGGCCCGGCTAAAGCGCTTTTG
CGCGCTATTGCTAACGATCCTCAACATGTCATCCCTGCGCTATCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66