Detailed information of TA system TA199253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 335836..336737
• Replicon: plasmid unnamed1
• Accession: NZ_CP072946
• Organism: Enterobacter sp. MGH 7

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A330G4J7
• Locus tag: IAU58_RS25375
• Protein ID: WP_022652120.1
• Coordinates: 335836..336294 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IAU58_RS25380
• Protein ID: WP_022652121.1
• Coordinates: 336291..336737 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU58_RS25340	331156..331791	+	636	WP_023279901.1	hypothetical protein	-
IAU58_RS25345	331819..332874	-	1056	WP_022652116.1	phosphoadenosine phosphosulfate reductase family protein	-
IAU58_RS25350	333052..333498	-	447	WP_015063027.1	hypothetical protein	-
IAU58_RS25355	333545..334351	-	807	WP_015063028.1	hypothetical protein	-
IAU58_RS25360	334581..334844	-	264	WP_032610382.1	hypothetical protein	-
IAU58_RS25365	334901..335233	-	333	WP_022652118.1	hypothetical protein	-
IAU58_RS25370	335568..335798	-	231	WP_022652119.1	hypothetical protein	-
IAU58_RS25375	335836..336294	-	459	WP_022652120.1	RES family NAD+ phosphorylase	Toxin
IAU58_RS25380	336291..336737	-	447	WP_022652121.1	DUF2384 domain-containing protein	Antitoxin
IAU58_RS25385	336814..337122	-	309	WP_022652122.1	hypothetical protein	-
IAU58_RS25390	337298..338566	-	1269	WP_022652123.1	DNA methyltransferase	-
IAU58_RS25395	338553..339164	-	612	WP_022652124.1	hypothetical protein	-
IAU58_RS25400	339226..339861	-	636	WP_022652125.1	hypothetical protein	-
IAU58_RS25405	339977..340234	-	258	WP_022652126.1	hypothetical protein	-
IAU58_RS25410	340298..340732	-	435	WP_015063073.1	hypothetical protein	-
IAU58_RS25415	340868..341245	-	378	WP_015063074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-30 / blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A	-	1..417086	417086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16758.14 Da
• Isoelectric Point: 4.4843

>T199253 WP_022652120.1 NZ_CP072946:c336294-335836 [Enterobacter sp. MGH 7]
VILYRMTKTRYITTAWSGLGAKEAGGRWNSVGTALVYLSETASLTMLETLVHINAPQLLDAYTLLSIDVPDDQIQAFDLN
LLPPGWASEDAPAELALYGDNWAESGSSVALRIPSALSPVEFNYLLNPAHPAIFDLMKTVQKIPYQYDTRLK

Nucleotide

• Length: 459 bp

>T199253 NZ_CP097255:c2721109-2721006 [Enterobacter bugandensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16606.03 Da
• Isoelectric Point: 7.4048

>AT199253 WP_022652121.1 NZ_CP072946:c336737-336291 [Enterobacter sp. MGH 7]
MTMKVFSPDLSKMNPHGLWQVVGLDNDGIALMDKISHGLEGKVAHSISEWANITPSELRKMSGIPNTTFNRSVKARFTAD
QSERLVRIIRVIERAVELFEGDKEAAQKWMNEPNRGLSWKTPADVVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT199253 NZ_CP097255:2721002-2721144 [Enterobacter bugandensis]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A330G4J7