Detailed information of TA system TA199249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3200441..3202224
• Replicon: chromosome
• Accession: NZ_CP072945
• Organism: Enterobacter sp. MGH 7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IAU58_RS15425
• Protein ID: WP_032609694.1
• Coordinates: 3200441..3201790 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: IAU58_RS15430
• Protein ID: WP_003859317.1
• Coordinates: 3201787..3202224 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU58_RS15400	3196264..3197199	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
IAU58_RS15405	3197368..3197763	+	396	WP_003859308.1	CidA/LrgA family protein	-
IAU58_RS15410	3197760..3198455	+	696	WP_003859310.1	CidB/LrgB family autolysis modulator	-
IAU58_RS15415	3198584..3199468	+	885	WP_032609693.1	cytidine deaminase	-
IAU58_RS15420	3199596..3200306	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
IAU58_RS15425	3200441..3201790	+	1350	WP_032609694.1	HipA domain-containing protein	Toxin
IAU58_RS15430	3201787..3202224	+	438	WP_003859317.1	helix-turn-helix transcriptional regulator	Antitoxin
IAU58_RS15435	3202263..3203273	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
IAU58_RS15440	3203289..3204809	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
IAU58_RS15445	3204889..3205887	-	999	WP_003859322.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
IAU58_RS15450	3206183..3207205	-	1023	WP_003859324.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51149.84 Da
• Isoelectric Point: 4.6562

>T199249 WP_032609694.1 NZ_CP072945:3200441-3201790 [Enterobacter sp. MGH 7]
METLTVQAYLNETWTDIALIKYPGSEKNDWNITQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHEINAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T199249 NZ_CP097252:5856157-5856429 [Mesorhizobium opportunistum]
ATGCTGACGCCCGTTCGCTCCGGGCAATTCCGCAGGGATGTGAAGCGTATGGAGAAACGCGGCAAAGACCTTGCTAAGTT
GCGCGAGCTGCTGGCCCTACTGGTCGCCGGGCAGGAACTGCCGGCGATTTACAAGGATCACCCTCTGAAAGGTGATTGGA
AGGGCTATCGTGACGCGCACGTGGAACCGGATTGGCTCTTGATCTACCGCATTGCCGACGATGAACTGCGGCTTGCCCGC
ACCGGCTCCCACTCCGATTTATTCAACGAGTGA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT199249 WP_003859317.1 NZ_CP072945:3201787-3202224 [Enterobacter sp. MGH 7]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT199249 NZ_CP097252:5855898-5856155 [Mesorhizobium opportunistum]
ATGGCAACGGATACGGTGGTGCGCGCTCGGATCGATACGGCGACAAAGGATCAAGCTACGGAAGCCTTGGCGGCCATGGG
ATTATCGGTTTCCGACGCCATTCGTCTACTGCTGGTCCGCGTCGCTGCCGACAAGGAATTTCCCTTTCCGGTGAAGGTGC
CGAACGCGACCACTCGGAAGGCAATGGCCGAATTGGAAAAAGGCAAGGGCGAGCGTTTCGCCTCGGCCAATGAGTTGTTC
AAGGATTTGGGCATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FFV9