Detailed information of TA system TA199236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 300285..300881
• Replicon: chromosome
• Accession: NZ_CP072945
• Organism: Enterobacter sp. MGH 7

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: IAU58_RS01440
• Protein ID: WP_023315213.1
• Coordinates: 300579..300881 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IAU58_RS01435
• Protein ID: WP_032610152.1
• Coordinates: 300285..300572 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU58_RS01430	298657..300288	+	1632	WP_032610151.1	Na/Pi cotransporter family protein	-
IAU58_RS01435	300285..300572	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
IAU58_RS01440	300579..300881	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAU58_RS01445	301079..301951	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
IAU58_RS01450	301952..302224	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
IAU58_RS01455	302275..303219	-	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
IAU58_RS01460	303313..304662	-	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T199236 WP_023315213.1 NZ_CP072945:c300881-300579 [Enterobacter sp. MGH 7]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T199236 NZ_CP097251:1678722-1679057 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTACTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCAGCCATTATCACAGCACTAAAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGCTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT199236 WP_032610152.1 NZ_CP072945:c300572-300285 [Enterobacter sp. MGH 7]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT199236 NZ_CP097251:1678445-1678732 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGACGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8