Detailed information of TA system TA199230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 330977..331575
• Replicon: chromosome
• Accession: NZ_CP072934
• Organism: Corynebacterium pseudogenitalium strain CCUG 27540

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: KBP54_RS01480
• Protein ID: WP_256006093.1
• Coordinates: 331267..331575 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1F1YMH8
• Locus tag: KBP54_RS01475
• Protein ID: WP_070362126.1
• Coordinates: 330977..331270 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP54_RS01450 (KBP54_01450)	326590..327267	-	678	WP_071569465.1	TetR/AcrR family transcriptional regulator	-
KBP54_RS01455 (KBP54_01455)	327359..328588	+	1230	WP_070495012.1	MFS transporter	-
KBP54_RS01460 (KBP54_01460)	328589..328729	-	141	WP_232745367.1	hypothetical protein	-
KBP54_RS01465 (KBP54_01465)	328845..329621	-	777	WP_256006091.1	ATP-binding protein	-
KBP54_RS01470 (KBP54_01470)	329915..330199	-	285	WP_143110101.1	hypothetical protein	-
KBP54_RS01475 (KBP54_01475)	330977..331270	-	294	WP_070362126.1	putative addiction module antidote protein	Antitoxin
KBP54_RS01480 (KBP54_01480)	331267..331575	-	309	WP_256006093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBP54_RS01485	331739..331870	+	132	WP_255364819.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	297097..340787	43690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-97)

Antitoxin

• Uncharacterized protein HI1420 (15-94)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11540.25 Da
• Isoelectric Point: 9.8180

>T199230 WP_256006093.1 NZ_CP072934:c331575-331267 [Corynebacterium pseudogenitalium]
VEIISSSLFDAWLGELKDKHAQRRILYAIARCETHGRMIGDIKPVGEKVSEMRFHFGPGYRVYYTRLGAITVFLLAGGDK
SSQAKDIRTAQKLAQQVREEQS

Nucleotide

• Length: 309 bp

>T199230 NZ_CP097248:2469197-2469565 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10578.05 Da
• Isoelectric Point: 7.0095

>AT199230 WP_070362126.1 NZ_CP072934:c331270-330977 [Corynebacterium pseudogenitalium]
VKEVTFSAFDASKYLDSQEAVNDYLAIALEDGDTKTVQVVLRDIARAHGMSQLAKETQLNRESLYKSLSKDGNPSFAAIT
KIMKALGLKITLATQNA

Nucleotide

• Length: 294 bp

>AT199230 NZ_CP097248:2468979-2469200 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1F1YMH8