Detailed information of TA system TA199228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1627147..1627669
• Replicon: chromosome
• Accession: NZ_CP072933
• Organism: Xylella fastidiosa subsp. multiplex strain AlmaEM3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A060HA53
• Locus tag: KBP49_RS07185
• Protein ID: WP_038229230.1
• Coordinates: 1627382..1627669 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A060HDF9
• Locus tag: KBP49_RS07180
• Protein ID: WP_004085758.1
• Coordinates: 1627147..1627389 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP49_RS07145 (KBP49_07145)	1622235..1622978	+	744	WP_225621865.1	polysaccharide deacetylase family protein	-
KBP49_RS07150 (KBP49_07150)	1623622..1623813	-	192	WP_027700471.1	hypothetical protein	-
KBP49_RS07155 (KBP49_07155)	1624231..1624650	-	420	WP_057683757.1	putative toxin-antitoxin system toxin component, PIN family	-
KBP49_RS07160 (KBP49_07160)	1624650..1624889	-	240	WP_004085761.1	ribbon-helix-helix domain-containing protein	-
KBP49_RS07165 (KBP49_07165)	1625273..1626307	+	1035	WP_071869505.1	hypothetical protein	-
KBP49_RS07170 (KBP49_07170)	1626426..1626677	+	252	Protein_1418	conjugal transfer protein TrbJ	-
KBP49_RS11055	1626630..1626857	-	228	WP_233341840.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
KBP49_RS07180 (KBP49_07180)	1627147..1627389	+	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
KBP49_RS07185 (KBP49_07185)	1627382..1627669	+	288	WP_038229230.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBP49_RS07190 (KBP49_07190)	1627757..1628323	+	567	WP_071869506.1	recombinase family protein	-
KBP49_RS07195 (KBP49_07195)	1628340..1629572	-	1233	WP_140160997.1	RNA-guided endonuclease TnpB family protein	-
KBP49_RS07200 (KBP49_07200)	1629566..1630192	-	627	WP_140160998.1	IS607 family transposase	-
KBP49_RS07205 (KBP49_07205)	1630422..1631696	-	1275	WP_012337985.1	pyridoxal phosphate-dependent aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1623828..1646934	23106
	-	flank	IS/Tn	-	-	1627757..1628323	566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11163.63 Da
• Isoelectric Point: 5.9570

>T199228 WP_038229230.1 NZ_CP072933:1627382-1627669 [Xylella fastidiosa subsp. multiplex]
MAKSYRLTPLAEADLEEIWFYTFRHWSIEQADSYHRSLVAVFEGLAAGTKQGCPSVLTDFNKYLCGSHVVYFMDYADHLD
IIRILHQRQDTERYL

Nucleotide

• Length: 288 bp

>T199228 NZ_CP097248:c1561333-1561049 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8774.77 Da
• Isoelectric Point: 6.9881

>AT199228 WP_004085758.1 NZ_CP072933:1627147-1627389 [Xylella fastidiosa subsp. multiplex]
MSRNTSVALGPHFTSFIDAQVQGGRYGTASDVVRAGLRLLEEHETKVKALQDALNVGHQSGEPRPFDSEVFLSRMHTQHG

Nucleotide

• Length: 243 bp

>AT199228 NZ_CP097248:c1561565-1561323 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A060HA53

Antitoxin

• AlphaFold DB: A0A060HDF9