Detailed information of TA system TA199226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1346850..1347455
• Replicon: chromosome
• Accession: NZ_CP072933
• Organism: Xylella fastidiosa subsp. multiplex strain AlmaEM3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: KBP49_RS05810
• Protein ID: WP_004089252.1
• Coordinates: 1346850..1347146 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KBP49_RS05815
• Protein ID: WP_038211623.1
• Coordinates: 1347150..1347455 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP49_RS05785 (KBP49_05785)	1342024..1342992	+	969	WP_004084992.1	hypothetical protein	-
KBP49_RS05790 (KBP49_05790)	1343251..1343697	+	447	WP_004084994.1	hypothetical protein	-
KBP49_RS05795 (KBP49_05795)	1343801..1345045	-	1245	WP_071869745.1	tail fiber protein	-
KBP49_RS05800 (KBP49_05800)	1345049..1345609	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
KBP49_RS05805 (KBP49_05805)	1345606..1346751	-	1146	WP_140160953.1	baseplate J/gp47 family protein	-
KBP49_RS05810 (KBP49_05810)	1346850..1347146	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBP49_RS05815 (KBP49_05815)	1347150..1347455	+	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
KBP49_RS05820 (KBP49_05820)	1347466..1347819	-	354	WP_071870030.1	hypothetical protein	-
KBP49_RS05825 (KBP49_05825)	1347816..1348457	-	642	WP_071870029.1	Gp138 family membrane-puncturing spike protein	-
KBP49_RS05830 (KBP49_05830)	1348454..1349284	-	831	WP_140160954.1	hypothetical protein	-
KBP49_RS05835 (KBP49_05835)	1349281..1349598	-	318	WP_012382585.1	hypothetical protein	-
KBP49_RS05840 (KBP49_05840)	1349598..1350368	-	771	WP_140160955.1	hypothetical protein	-
KBP49_RS05845 (KBP49_05845)	1350479..1350706	+	228	WP_004091377.1	DUF6290 family protein	-
KBP49_RS05850 (KBP49_05850)	1350690..1350956	+	267	WP_004087087.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1343801..1363808	20007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T199226 WP_004089252.1 NZ_CP072933:1346850-1347146 [Xylella fastidiosa subsp. multiplex]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T199226 NZ_CP097248:848954-849172 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT199226 WP_038211623.1 NZ_CP072933:1347150-1347455 [Xylella fastidiosa subsp. multiplex]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT199226 NZ_CP097248:848553-848927 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTAGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A060HBK9