Detailed information of TA system TA199223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 473365..473951
• Replicon: chromosome
• Accession: NZ_CP072933
• Organism: Xylella fastidiosa subsp. multiplex strain AlmaEM3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q87BV5
• Locus tag: KBP49_RS01970
• Protein ID: WP_004089517.1
• Coordinates: 473697..473951 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: KBP49_RS01965
• Protein ID: WP_004085025.1
• Coordinates: 473365..473700 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP49_RS01935 (KBP49_01935)	470143..470676	-	534	WP_051247998.1	terminase small subunit	-
KBP49_RS01940 (KBP49_01940)	470849..471067	-	219	WP_247644821.1	hypothetical protein	-
KBP49_RS01945 (KBP49_01945)	471075..471536	-	462	WP_140160960.1	hypothetical protein	-
KBP49_RS01950 (KBP49_01950)	471526..471855	-	330	WP_004085022.1	phage holin family protein	-
KBP49_RS01955 (KBP49_01955)	471848..472348	-	501	WP_154195470.1	lysozyme	-
KBP49_RS01960 (KBP49_01960)	472443..473175	-	733	Protein_372	site-specific DNA-methyltransferase	-
KBP49_RS01965 (KBP49_01965)	473365..473700	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KBP49_RS01970 (KBP49_01970)	473697..473951	-	255	WP_004089517.1	type II toxin-antitoxin system HicA family toxin	Toxin
KBP49_RS01975 (KBP49_01975)	474031..474726	-	696	WP_140162110.1	hypothetical protein	-
KBP49_RS01980 (KBP49_01980)	475202..476470	+	1269	WP_247644822.1	DNA polymerase	-
KBP49_RS01985 (KBP49_01985)	476467..476745	+	279	WP_071869551.1	VRR-NUC domain-containing protein	-
KBP49_RS01990 (KBP49_01990)	476747..477052	-	306	WP_024749221.1	hypothetical protein	-
KBP49_RS01995 (KBP49_01995)	477138..478556	+	1419	WP_071869550.1	DEAD/DEAH box helicase	-
KBP49_RS02000 (KBP49_02000)	478553..478717	+	165	WP_162848953.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	433775..482474	48699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9639.14 Da
• Isoelectric Point: 11.2766

>T199223 WP_004089517.1 NZ_CP072933:c473951-473697 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T199223 NZ_CP097245:c2865467-2865069 [Leptospira santarosai]
ATGTATCTACTTGATACGAATATTTGTATCTTTCTCATTAAAAAGAAGAGTCAAAAATTATTAGATAAATTAAAGAAGAA
CTTCAACAAAGGTCTGTATATTTCATCGCTAACTTTGGCTGAATTGGAATTTGGCATTGAGAATAGTGAACAAAAAGAAA
AAAATAGGGTATCGTTAATTGAATTTCTTTCCATATTTGAAATTCTTCCGTTTGAGCAATCTGATGCACAAGCATTCGGA
ATTCTTAAGGCTGATTTAAAGAAATCAGGTAATCCTATTGGATCCATTGACGCTCTATTGGCAGCCCAGGCATTGTCGAG
AGTTTTAGTATTGATTACTAATAATACTAAAGAATTTAAGAGAGTAAAGAATCTCAGCATTGAAGATTGGTCTCAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT199223 WP_004085025.1 NZ_CP072933:c473700-473365 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT199223 NZ_CP097245:c2865697-2865467 [Leptospira santarosai]
ATGCACACTGCAAAGTTATTTATTAACGGAAGAAGTCAAGCTGTAAGACTTCCAAAAGAATTTCAGTTTAAAGGTGACGA
TGTTTTTATTCAAAAAATAGGTGAAGCTGTAATTTTAGTTCCTAAGAATAAAGCGTGGAACGTTTTCCTCGATGGACTTA
ACGGATTTACTGAAGATTTTCTAAAAGAAGGAAGAGAAGGGCGCCCCGAAACTGAAAGAGAAAAATTTTAA

Structures

Toxin

• AlphaFold DB: Q87BV5

Antitoxin

• AlphaFold DB: A0A060HAK5