Detailed information of TA system TA199219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1034562..1035167
• Replicon: chromosome
• Accession: NZ_CP072932
• Organism: Xylella fastidiosa subsp. multiplex strain BB08-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: KBP48_RS04745
• Protein ID: WP_004089252.1
• Coordinates: 1034871..1035167 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KBP48_RS04740
• Protein ID: WP_038211623.1
• Coordinates: 1034562..1034867 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP48_RS04705 (KBP48_04705)	1031061..1031327	-	267	WP_004087087.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KBP48_RS04710 (KBP48_04710)	1031311..1031538	-	228	WP_004091377.1	DUF6290 family protein	-
KBP48_RS04715 (KBP48_04715)	1031649..1032419	+	771	WP_140160955.1	hypothetical protein	-
KBP48_RS04720 (KBP48_04720)	1032419..1032736	+	318	WP_012382585.1	hypothetical protein	-
KBP48_RS04725 (KBP48_04725)	1032733..1033563	+	831	WP_038230303.1	hypothetical protein	-
KBP48_RS04730 (KBP48_04730)	1033560..1034201	+	642	WP_140189635.1	Gp138 family membrane-puncturing spike protein	-
KBP48_RS04735 (KBP48_04735)	1034198..1034551	+	354	WP_020851652.1	hypothetical protein	-
KBP48_RS04740 (KBP48_04740)	1034562..1034867	-	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
KBP48_RS04745 (KBP48_04745)	1034871..1035167	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBP48_RS04750 (KBP48_04750)	1035266..1036411	+	1146	WP_140189634.1	baseplate J/gp47 family protein	-
KBP48_RS04755 (KBP48_04755)	1036408..1036968	+	561	WP_004084996.1	DUF2612 domain-containing protein	-
KBP48_RS04760 (KBP48_04760)	1036972..1038216	+	1245	WP_140189633.1	tail fiber protein	-
KBP48_RS04765 (KBP48_04765)	1038320..1038766	-	447	WP_004084994.1	hypothetical protein	-
KBP48_RS04770 (KBP48_04770)	1039025..1039993	-	969	WP_004084992.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1017991..1038216	20225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T199219 WP_004089252.1 NZ_CP072932:c1035167-1034871 [Xylella fastidiosa subsp. multiplex]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T199219 NZ_CP097245:440032-440217 [Leptospira santarosai]
ATGAAAGTAAAAGAGATAATCAAAATTTTAGAGGATGACGGTTGGTATTTGGTAGATCAGAGAGGAAGTCACAGGCAGTT
TAAGCATAGCTTAAAATACGGGAGAGTAACTGTAGCGGGAAAGCCAAGTATGGATATACCTCCCGGGACTTTGAATAGTA
TCTTAAAACAATCCGGTCTAAAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT199219 WP_038211623.1 NZ_CP072932:c1034867-1034562 [Xylella fastidiosa subsp. multiplex]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT199219 NZ_CP097245:440234-440437 [Leptospira santarosai]
ATGGAATACCCAGTAATGATTGAAAAGAGTTCCACGGGTTATGGAGCGTATGTCCCGGATTTACCCGGCTGTGTGGCGGT
TGGAGAGACGGAAGCGGAAGTAACTCGTTTAATCAAGGAAGCGATAGAGTTTCATTTAGAAGGTATGCGTAAAGATGGAG
AACCGATTCCGAATCCTTCTAAGGTAACCTTAATAGCGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9