Detailed information of TA system TA199215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2599825..2600389
• Replicon: chromosome
• Accession: NZ_CP072931
• Organism: Streptomyces auratus AGR0001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SU9_RS11055
• Protein ID: WP_246593866.1
• Coordinates: 2599825..2600151 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2JYC8
• Locus tag: SU9_RS11060
• Protein ID: WP_006605605.1
• Coordinates: 2600159..2600389 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SU9_RS11040 (SU9_011040)	2595468..2596490	+	1023	WP_006605609.1	hypothetical protein	-
SU9_RS11045 (SU9_011045)	2596549..2598294	-	1746	WP_006605608.1	TIGR03767 family metallophosphoesterase	-
SU9_RS11050 (SU9_011050)	2598700..2599731	+	1032	WP_006605607.1	aspartate-semialdehyde dehydrogenase	-
SU9_RS11055 (SU9_011055)	2599825..2600151	-	327	WP_246593866.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SU9_RS11060 (SU9_011060)	2600159..2600389	-	231	WP_006605605.1	ribbon-helix-helix domain-containing protein	Antitoxin
SU9_RS11065 (SU9_011065)	2600514..2603102	+	2589	WP_006605604.1	aminopeptidase N	-
SU9_RS11070 (SU9_011070)	2603180..2604331	-	1152	WP_006605603.1	allantoicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11953.77 Da
• Isoelectric Point: 10.8989

>T199215 WP_246593866.1 NZ_CP072931:c2600151-2599825 [Streptomyces auratus AGR0001]
MIDLEPARGSEANKTRPAVIVSNNGANQSVESNRRGVVTVVPLTSSTSRVLTFQVFLGAEECRLPKDCKAQCEQVRAVTV
ERVRQRVGSVPRQRMVEIDVALRRHLAL

Nucleotide

• Length: 327 bp

>T199215 NZ_CP097238:128834-129304 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8450.13 Da
• Isoelectric Point: 3.7951

>AT199215 WP_006605605.1 NZ_CP072931:c2600389-2600159 [Streptomyces auratus AGR0001]
MKISVSLPQEDLAFVDEYATRTEAESRSAVIHAAIELLRAAELESEYTEAFEEWDASEDAALWDRTSGDGLADEAR

Nucleotide

• Length: 231 bp

>AT199215 NZ_CP097238:128394-128837 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: J2JYC8