Detailed information of TA system TA199212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX(toxin)
• Location: 4606776..4607595
• Replicon: chromosome
• Accession: NZ_CP072914
• Organism: Klebsiella oxytoca isolate KoPF10

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: J5WT09
• Locus tag: KAT64_RS21745
• Protein ID: WP_004110819.1
• Coordinates: 4607338..4607595 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: KAT64_RS21740
• Protein ID: WP_210669405.1
• Coordinates: 4606776..4607327 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAT64_RS21720 (KAT64_21720)	4601846..4602394	-	549	WP_004099233.1	type 1 fimbrial major subunit FimA	-
KAT64_RS21725 (KAT64_21725)	4602767..4603534	+	768	WP_004099232.1	winged helix-turn-helix domain-containing protein	-
KAT64_RS21730 (KAT64_21730)	4604162..4604839	+	678	WP_135800006.1	sigma-70 region 4 domain-containing protein	-
KAT64_RS27520	4605130..4605894	-	765	WP_224227635.1	class I SAM-dependent methyltransferase	-
KAT64_RS27525	4606270..4606662	-	393	Protein_4291	class I SAM-dependent methyltransferase	-
KAT64_RS21740 (KAT64_21740)	4606776..4607327	-	552	WP_210669405.1	N-acetyltransferase	Antitoxin
KAT64_RS21745 (KAT64_21745)	4607338..4607595	-	258	WP_004110819.1	YjhX family toxin	Toxin
KAT64_RS21750 (KAT64_21750)	4608176..4609360	+	1185	WP_004099225.1	mannonate dehydratase	-
KAT64_RS21755 (KAT64_21755)	4609435..4610910	+	1476	WP_210669406.1	fructuronate reductase	-
KAT64_RS21760 (KAT64_21760)	4611046..4611822	+	777	WP_004099223.1	Uxu operon transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (17-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9552.07 Da
• Isoelectric Point: 11.1381

>T199212 WP_004110819.1 NZ_CP072914:c4607595-4607338 [Klebsiella oxytoca]
MNLSRQEQRTLHVLAKGGRIAHIRDASGRVTSVECYSREGLLLSDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T199212 NZ_CP097237:3148605-3148922 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20158.97 Da
• Isoelectric Point: 6.8799

>AT199212 WP_210669405.1 NZ_CP072914:c4607327-4606776 [Klebsiella oxytoca]
MTNHNFTFHITSERDADDIREVETRAFGFSKEADLVAALLNDESAHSSLSLLAKHNGKAVGHILFTLATFKGESDSPMMH
ILAPLAVVPEYQGVGVGGLLIQRGIEHLKAAGSEAVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMLQRLSS
RPLGRTGQIQCARALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT199212 NZ_CP097237:3148915-3149202 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H6Y2