Detailed information of TA system TA199211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4367971..4368590
• Replicon: chromosome
• Accession: NZ_CP072914
• Organism: Klebsiella oxytoca isolate KoPF10

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: KAT64_RS20625
• Protein ID: WP_004099646.1
• Coordinates: 4368372..4368590 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KAT64_RS20620
• Protein ID: WP_004099648.1
• Coordinates: 4367971..4368345 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAT64_RS20610 (KAT64_20610)	4363127..4364320	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KAT64_RS20615 (KAT64_20615)	4364343..4367489	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit AcrB	-
KAT64_RS20620 (KAT64_20620)	4367971..4368345	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
KAT64_RS20625 (KAT64_20625)	4368372..4368590	+	219	WP_004099646.1	HHA domain-containing protein	Toxin
KAT64_RS20630 (KAT64_20630)	4368751..4369317	+	567	WP_004099644.1	maltose O-acetyltransferase	-
KAT64_RS27510	4369286..4369423	-	138	WP_224226720.1	hypothetical protein	-
KAT64_RS20635 (KAT64_20635)	4369454..4369924	+	471	WP_004111038.1	YlaC family protein	-
KAT64_RS20640 (KAT64_20640)	4369899..4371353	-	1455	Protein_4072	PLP-dependent aminotransferase family protein	-
KAT64_RS20645 (KAT64_20645)	4371456..4372154	+	699	WP_004099639.1	GNAT family protein	-
KAT64_RS20650 (KAT64_20650)	4372151..4372291	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
KAT64_RS20655 (KAT64_20655)	4372291..4372554	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T199211 WP_004099646.1 NZ_CP072914:4368372-4368590 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T199211 NZ_CP097237:c3024532-3024314 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT199211 WP_004099648.1 NZ_CP072914:4367971-4368345 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT199211 NZ_CP097237:c3024933-3024559 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3H713