Detailed information of TA system TA199202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2009638..2010228
• Replicon: chromosome
• Accession: NZ_CP072914
• Organism: Klebsiella oxytoca isolate KoPF10

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KAT64_RS09435
• Protein ID: WP_061372072.1
• Coordinates: 2009896..2010228 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KAT64_RS09430
• Protein ID: WP_070557012.1
• Coordinates: 2009638..2009895 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAT64_RS09415 (KAT64_09415)	2006305..2008260	-	1956	WP_070557018.1	hypothetical protein	-
KAT64_RS09420 (KAT64_09420)	2008614..2009087	+	474	WP_070557016.1	hypothetical protein	-
KAT64_RS09425 (KAT64_09425)	2009084..2009290	+	207	WP_070557014.1	helix-turn-helix transcriptional regulator	-
KAT64_RS09430 (KAT64_09430)	2009638..2009895	+	258	WP_070557012.1	antitoxin	Antitoxin
KAT64_RS09435 (KAT64_09435)	2009896..2010228	+	333	WP_061372072.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAT64_RS09440 (KAT64_09440)	2011074..2011958	+	885	WP_070557010.1	integrase domain-containing protein	-
KAT64_RS09445 (KAT64_09445)	2012656..2013918	-	1263	WP_210669699.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1998196..2037096	38900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11816.58 Da
• Isoelectric Point: 9.3417

>T199202 WP_061372072.1 NZ_CP072914:2009896-2010228 [Klebsiella oxytoca]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T199202 NZ_CP097233:164648-165130 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9310.78 Da
• Isoelectric Point: 4.5821

>AT199202 WP_070557012.1 NZ_CP072914:2009638-2009895 [Klebsiella oxytoca]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEESREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT199202 NZ_CP097233:164379-164657 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA