Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 7055..7548 | Replicon | plasmid pKpCol17mcr |
| Accession | NZ_CP072908 | ||
| Organism | Klebsiella pneumoniae strain COL17 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9Y9W8 |
| Locus tag | GQ627_RS28705 | Protein ID | WP_001230709.1 |
| Coordinates | 7055..7309 (+) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | U9Y4R8 |
| Locus tag | GQ627_RS28710 | Protein ID | WP_000151456.1 |
| Coordinates | 7306..7548 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GQ627_RS29320 (GQ627_0mcr00003) | 2570..2722 | + | 153 | Protein_2 | DUF5431 family protein | - |
| GQ627_RS28670 (GQ627_0mcr00003) | 2670..2789 | + | 120 | WP_228932012.1 | Hok/Gef family protein | - |
| GQ627_RS28675 (GQ627_0mcr00004) | 2939..3535 | + | 597 | WP_000125165.1 | ProQ/FINO family protein | - |
| GQ627_RS28680 (GQ627_0mcr00005) | 3553..3837 | - | 285 | WP_001162371.1 | hypothetical protein | - |
| GQ627_RS28685 (GQ627_0mcr00006) | 3911..4576 | - | 666 | WP_000532148.1 | division plane positioning ATPase MipZ | - |
| GQ627_RS28690 (GQ627_0mcr00007) | 5164..5493 | + | 330 | WP_000855119.1 | hypothetical protein | - |
| GQ627_RS28695 (GQ627_0mcr00008) | 5897..6412 | + | 516 | WP_001025393.1 | J domain-containing protein | - |
| GQ627_RS28700 (GQ627_0mcr00009) | 6516..7058 | + | 543 | WP_001083904.1 | hypothetical protein | - |
| GQ627_RS28705 (GQ627_0mcr00010) | 7055..7309 | + | 255 | WP_001230709.1 | hypothetical protein | Toxin |
| GQ627_RS28710 (GQ627_0mcr00011) | 7306..7548 | + | 243 | WP_000151456.1 | hypothetical protein | Antitoxin |
| GQ627_RS28715 (GQ627_0mcr00012) | 8119..8412 | + | 294 | WP_000673428.1 | hypothetical protein | - |
| GQ627_RS29325 (GQ627_0mcr00014) | 8829..9272 | - | 444 | WP_000192625.1 | hypothetical protein | - |
| GQ627_RS28725 (GQ627_0mcr00015) | 9569..9751 | + | 183 | WP_000833470.1 | type II toxin-antitoxin system HicA family toxin | - |
| GQ627_RS28730 (GQ627_0mcr00016) | 9776..10213 | + | 438 | WP_000466318.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| GQ627_RS29330 | 10347..10532 | - | 186 | Protein_16 | hypothetical protein | - |
| GQ627_RS28740 (GQ627_0mcr00018) | 10672..11112 | - | 441 | WP_104652140.1 | hypothetical protein | - |
| GQ627_RS28745 (GQ627_0mcr00019) | 11132..11839 | - | 708 | WP_000108794.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | mcr-1.1 | - | 1..60525 | 60525 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 9942.47 Da Isoelectric Point: 10.1206
>T199194 WP_001230709.1 NZ_CP072908:7055-7309 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
Download Length: 255 bp
>T199194 NZ_CP097232:2112403-2112621 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9136.45 Da Isoelectric Point: 7.2775
>AT199194 WP_000151456.1 NZ_CP072908:7306-7548 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
Download Length: 243 bp
>AT199194 NZ_CP097232:2112002-2112376 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9H8F0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9GNF5 |